ISSN:
1434-0879
Keywords:
MDCK cells
;
Allopurinol
;
Shock waves
;
Lithotripsy
;
Calcium signalling
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Allopurinol has been reported to ameliorate the side effects in patients following shock wave lithotripsy (SWL); however, the mechanism has not been studied. We have examined the protective effect of allopurinol on Madin-Darby canine kidney (MDCK) cells after shock wave exposure (SWE) by determining the release of aspartate aminotransferase (ASAT) and lactate dehydrogenase (LD), and the resting cytosolic Ca2+ concentration ([Ca2+]i). In SWE-treated cells, the release of ASAT and LD increased immediately, but largely transiently, by approximately 23% and 5-fold over control, respectively. Within 1–6 h after SWE there was a gradual rise in the resting [Ca2+]i of 16–137% above control. Allopurinol did not affect the transient enzyme release but blocked the long-term rises in the resting [Ca2+]i. The transient changes in [Ca2+]i evoked by two hormones, ATP and bradykinin, and a drug that releases Ca2+ from internal Ca2+ stores, thapsigargin, were only slightly affected in allopurinol-treated cells. We conclude that the protection conferred by allopurinol on patients treated with SWL might involve a direct protection of the kidney cells by maintaining a normal resting [Ca2+]i.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01268861
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