ISSN:
1432-2242
Keywords:
Key words Microsatellites
;
Variable number of tandem repeats (VNTRs)
;
Enriched library
;
Hexamer repeat
;
Null allele
;
Marker-assisted selection (MAS)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We report the development of microsatellites or simple sequence repeats (SSRs) in the olive tree (Olea europaea L.). Forty three positive clones obtained by the screening of a GA-enriched genomic library were sequenced and primers were designed for 13 microsatellite loci. Five primer pairs amplified polymorphic products of the expected size range. SSR polymorphism was explored in a set of 46 olive cultivars. A total of 26 alleles were detected for the five loci. Heterozygosity ranged from 0.46 to 0.71. Ninety one per cent of the cultivars had unique multilocus genotypes. Microsatellite segregation was studied in a complex population from a cross between the commercial cultivars ’Leccino’ and ’Dolce Agogia’.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s001220051571
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