ISSN:
1432-072X
Keywords:
Mixed substrate
;
Chemostat
;
Methanol
;
Hansenula polymorpha
;
Growth-rate limitation
;
Carbonflow
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The yeast Hansenula polymorpha was grown in a chemostat using either methanol or sorbitol as substrate or a mixture of both. Methanol alone could be utilized up to a dilution rate (D) of 0.18 h-1, and sorbitol allowed growth at D's higher than 0.52 h-1. In combination with sorbitol, methanol was completely utilized in the mixture even up to a D of 0.3 h-1, and partially utilized at higher D's, To elucidate the basis of methanol utilization at high D's, enzyme activities on the single substrates and on the substrate mixture were compared. At D's above 0.3 h-1 an increase of formate dehydrogenase activity was evident, an enzyme involved in the oxidation of methanol to carbon dioxide. It was concluded that at high D's large amounts of methanol were oxidized to generate energy. This was proved with 14C-methanol, and it was found that in the range of partial methanol utilization approximately 75% of methanol was converted to carbon dioxide and 25% incorporated into cell material.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00414601
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