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  • Microbial biomass  (1)
  • N-mineralization  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Biology and fertility of soils 8 (1989), S. 54-60 
    ISSN: 1432-0789
    Schlagwort(e): Biomass N ; Humus fractions ; Hydrolyzable N ; Legume N ; Mineralizable N ; N-mineralization ; 15N-labelled material
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Geologie und Paläontologie , Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Two soils from Pakistan (Hafizabad silt loam and Khurrarianwala silt loam) and one from Illinois, USA (Drummer silty clay loam) were incubated with 15N-labelled soybean tops for up to 20 weeks at 30°C. Mineralization of soybean 15N was slightly more rapid in the Pakistani soils, and after 20 weeks of incubation, 50%, 53%, and 56% of the applied 15N was accounted for as (NH4 ++NO3 −)-N in Drummer, Hafizabad, and Khurrarianwala soils, respectively. Potentially mineralizable N (determined by anaerobic incubation) varied between 1.5% and 10% of the applied 15N in the three soils at different stages of incubation; somewhat higher percentages were mineralizable in the Pakistani soils than in the Drummer soil. From 3.7% to 9% of the applied 15N was accounted for in the microbial biomass. From 10% to 32% of the applied N was recovered in the humic acid and fulvic acid fractions of the organic matter by sequential extraction with Na4P2O7 and NaOH; from 12% to 49% was recovered in the humin fraction. Of the three soils, Drummer soil contained more 15N as humic and fulvic acids. In all cases, the 15N was approximately equally distributed between the humic and fulvic acid fractions. A significant percentage of the humin 15N (52%–78%, equivalent to 8%–34% of the applied 15N) occurred in non-hydrolyzable (6 N HCl) forms. Of the hydrolyzable 15N, 42%–51% was accounted for as amino acid-N followed in order by NH3 (17%–30%), hydrolyzable unknown forms (20%–22%), and amino sugars (6%–2%). The recovery of applied 15N for the different incubation stages was 87±22%. Recovery was lowest with the Khurrarianwala soil, presumably because of NH3 volatilization losses caused by the high pH of this soil.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Biology and fertility of soils 7 (1989), S. 180-185 
    ISSN: 1432-0789
    Schlagwort(e): Extractability ratios ; Microbial biomass ; N immobilization-remineralization ; Priming effect
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Geologie und Paläontologie , Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary A Pakistani soil (Hafizabad silt loam) was incubated at 30°C with varying levels of 15N-labelled ammonium sulphate and glucose (C/N ratio of 30 at each addition rate) in order to generate different insitu levels of 15N-labelled microbial biomass. At a stage when all of the applied 15N was in organic forms, as biomass and products, the soil samples were analysed for biomass N by the chloroform (CHCl3) fumigation-extraction method, which involves exposure of the soil to CHCl3 vapour for 24 h followed by extraction with 500 mM K2SO4. A correction is made for inorganic and organic N in 500 mM K2SO4 extracts of the unfumigated soil. Results obtained using this approach were compared with the amounts of immobilized 15N extracted by 500 mM K2SO4 containing different amounts of CHCl3. The extraction time varied from 0.5 to 4 h. The amount of N extracted ranged from 27 to 270 μg g−1, the minimum occurring at the lowest (67 μg g−1) and the maximum at the highest (333 μg g−1) N-addition rate. Extractability of biomass 15N ranged from 25% at the lowest N-addition rate to 65%a for the highest rate and increased consistently with an increase in the amount of 15N and glucose added. The amounts of both soil N and immobilized 15N extracted with 500 mM K2SO4 containing CHCl3 increased with an increase in extraction time and in concentration of CHCl3. The chloroform fumigation-extraction method gives low estimates for biomass N because some of the organic N in K2SO4 extracts of unfumigated soil is derived from biomass.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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