ISSN:
1615-6102
Keywords:
Amiprophos-methyl
;
Cell shape
;
Colchicine
;
Fern protonema
;
Adiantum capillus-veneris L.
;
Microfibril
;
Microtubule
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary 5 mM colchicine and 1 μg/ml amiprophos-methyl, known antimicrotubule agents, were applied to fernAdiantum protonemata under red light. Both drugs caused microtubule disruption and subsequent apical swelling of protonemal cells after certain lag periods. While the lag periods for the onset of microtubule disruption after application of the two drugs were different (within 15 minutes in amiprophos-methyl, 1 hour in colchicine), the lag periods of apical swelling after microtubule disruption were nearly the same (approx. 70 minutes). The results suggest that the apical swelling is a consequence of microtubule disruption. In cells examined 1 hour after microtubule disruption by either drug, the microfibril arrangement of the innermost layer of the cell wall was random at the tip, transverse in the subapical region, and roughly longitudinal in the cylindrical region. This pattern of microfibrils was similar to that of untreated cells in which the microtubules show a similar arrangement (Murata and Wada 1989). Surprisingly, even after approx. 4 hours of microtubule disruption, when apical swelling had occurred in most cells, the pattern of microfibril deposition was not altered. The role of microtubules in oriented microfibril deposition and the mechanism of control of cell shape are discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01403444
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