ISSN:
1432-2242
Keywords:
Key words Rhododendron sp.
;
Flower colour
;
Leaf chlorosis
;
Molecular linkage map
;
Quantitative trait loci
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A molecular linkage map of Rhododendron has been constructed by using a segregating population from an interspecific cross. Parent-specific maps based on 239 RAPD, 38 RFLP, and two microsatellite markers were aligned using markers heterozygous in both parents. The map of the male parent ‘Cunningham’s White’ comprised 182 DNA markers in 13 linkage groups corresponding to the basic chromosome number. In the female parent ‘Rh 16’ 168 markers were located on 18 linkage groups. An assignment of putative homologous linkage groups was possible for 11 groups of each parent. QTL analyses based on the non-parametric Kruskal-Wallis rank-sum test were performed for the characters “leaf chlorosis” and “flower colour” scored as quantitative traits. For leaf chlorosis, two genomic regions bearing QTLs with significant effects on the trait were identified on two linkage groups of the chlorosis-tolerant parent. RAPD marker analysis of additional lime-stressed genotypes tested under altered environmental conditions verified the relationship between marker allele frequencies and the expression of chlorosis. Highly significant QTL effects for flower colour were found on two chromosomes indicating major genes located in these genome areas. The prospects for utilization of a linkage map in Rhododendron are discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s001220051179
Permalink
