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  • Autonomously replicating sequence  (1)
  • Mutagenesis  (1)
  • Phosphoprotein  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Multiple phosphorylated forms of the product of the fission yeast cell division cycle gene cdc2 + (1988)
    Springer
    Current genetics 14 (1988), S. 235-240 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Cell Cycle ; Phosphoprotein ; Schizosaccharomyces pombe
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The 34 kilodalton protein product (p34) of the cdc2 + cell cycle control gene of Schizosaccharomyces pombe was expressed in bacteria. Monoclonal antibodies raised against this protein are capable of immunoprecipitating p34dc2 from yeast lysates. Immunoprecipitates of [35S]methionine- and [32P]orthophosphate-labeled p34cdc2 were analyzed by two-dimensional gel electrophoresis. The cdc2 + gene product is homogeneous in size but resolves into seven species of differing charge. At least four of these species are phosphorylated. Phosphoamino acid analysis reveals that phosphorylation occurs mainly on threonine residues. The pattern of p34 phosphorylation is unaltered at the nonpermissive temperature in strains carrying temperature sensitive alleles of wee1-50 and ran1-114 or in a strain over-producing the ran1 + gene product.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00376743
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Two new multi-purpose multicopy Schizosaccharomyces pombe shuttle vectors, pSP1 and pSP2 (1993)
    Springer
    Current genetics 23 (1993), S. 547-548 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Autonomously replicating sequence ; Recombinant DNA ; Nested deletions ; Mutagenesis ; Schizosaccharomyces pombe
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Plasmids pSP1 and pSP2 are two new Schizosaccharomyces pombe ars1 multicopy vectors with the Saccharomyces cerevisiae LEU2 and URA3 genes as selectable markers. They are derivatives of S. cerevisiae integrative plasmids. These plasmids allow classical molecular genetic techniques, such as mutagenesis, nested deletions and sequencing, to be performed directly.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00312650
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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    Artikel (Nationallizenzen)
    Volltext
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