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  • Engineering General  (1)
  • Nonisotopic DNA probe  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    European journal of epidemiology 7 (1991), S. 77-82 
    ISSN: 1573-7284
    Keywords: Listeria monocytogenes ; Ribosomal DNA fingerprinting ; Nonisotopic DNA probe ; Digoxigenin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A nonisotopic ribosomal DNA fingerprinting technique was developed for the characterization of Listeria monocytogenes. Plasmid pKK3535 (a pBR322-derived plasmid containing rrnB ribosomal RNA operon of Escherichia coli) was labeled with digoxigenin-II-dUTP by random priming and used to probe EcoRI fragments of L. monocytogenes chromosomal DNA on nylon filters. The method was successfully applied to the characterization of two sets of patient and food isolates of L. monocytogenes.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 22 (1996), S. 283-296 
    ISSN: 0271-2091
    Keywords: laminar flow ; numerical modelling ; particle image velocimetry measurement ; rotating disc ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The fluid flow field within an enclosed cylindrical chamber with a rotating flat disc was calculated using a finite volume computational fluid dynamics (CFD) model and compared with particle image velocimetry (PIV) measurements. Two particular laminar cases near the Transitional flow regime were investigated: Reynolds number Re=2.5×1 4, chamber aspect ratio G (h/Rd)=0.2 and Re=4.2×104, G (h/Rd)=0.217. This enabled direct comparison with the numerical and experimental results reported by other researchers. The computational details and some major factors that affect the computed accuracy and convergence speed are also discussed in detail. PIV results containing some 4300 velocity vector points in each of seven planes for each case were obtained from the flow field parallel to the rotating disc. It was found that PIV results could be obtained in planes within the boundary layers as well as the core flow by careful use of a thin laser illumination sheet and correct choice of laser pulse separation. There was close agreement between numerical results, the present PIV measurements and other reported experimental and numerical results.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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