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  • 1
    Electronic Resource
    Electronic Resource
    Purification and properties of the adenylate kinases from Rhodopseudomonas palustris, Rhodopseudomonas sphaeroides and Rhodopseudomonas rubrum (1983)
    Springer
    Archives of microbiology 134 (1983), S. 153-157 
    Details
    ISSN: 1432-072X
    Keywords: Adenylate kinase ; Chromatophores ; Membranes ; Photosynthesis ; Rhodopseudomonas ; Rhodospirillum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The adenylate kinases (EC 2.7.4.3) from photosynthetically grown Rhodopseudomonas palustris, Rhodopseudomonas sphaeroides and Rhodospirillum rubrum were purified to homogeneity by the same procedure. The purified enzymes showed optimal rates of activity with MgCl2 at 25° C and pH 8.0. They were found to be heat labile and were characterized by pI-values of 4.5. Apparent molecular weights of 33 500 for R. palustris, 34 400 for R. sphaeroides and 32 100 for R. rubrum were determined by high performance liquid chromatography. No separation into subunits was observed by use of sodium dodecylsulfate polyacrylamide gel electrophoresis. The apparent K m -values for ADP corresponded to 0.26 mM for R. palustris, 0.27 mM for R. sphaeroides and 0.24 mM for R. rubrum. ADP in excess had a strong inhibitory effect. Competitive product inhibition was found for AMP, with K i-values of 0.017 mM for R. palustris, 0.018 mM for R. sphaeroides and 0.014 mM for R. rubrum. A competitive inhibitor likewise was P1,P5-di(adenosine-5′)pentaphosphate with K i-values of 0.020 μM for R. palustris and R. sphaeroides, and 0.017 μM for R. rubrum. Sulfhydryl-reacting reagents like p-chloromercuribenzoate and iodoacetic acid were found to be non-inhibitory. All measurements of adenylate kinase activity were carried out with the stabilized and most sensitive luciferin-luciferase system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00407949
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  • 2
    Electronic Resource
    Electronic Resource
    Chemical Effects of Nuclear Transformations in Solids (1967)
    Weinheim : Wiley-Blackwell
    Angewandte Chemie International Edition in English 6 (1967), S. 133-149 
    Details
    ISSN: 0570-0833
    Keywords: Solids ; Radiochemistry ; Nuclear chemistry ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Nuclear reactions and radioactive transformations offer the possibility of investigating the chemical reactions of “bare” nuclei. The interest in such chemical processes following nuclear transformations has already led to two international symposia[1,2]. In solids, the chemical reactions of recoil nuclei having energies lower than 1 keV proceed not via molten states, but via regions of little disorder.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/anie.196701331
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    Paper (German National Licenses)
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