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  • Listeria monocytogenes  (1)
  • Numerical modelling  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    European journal of epidemiology 7 (1991), S. 77-82 
    ISSN: 1573-7284
    Keywords: Listeria monocytogenes ; Ribosomal DNA fingerprinting ; Nonisotopic DNA probe ; Digoxigenin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A nonisotopic ribosomal DNA fingerprinting technique was developed for the characterization of Listeria monocytogenes. Plasmid pKK3535 (a pBR322-derived plasmid containing rrnB ribosomal RNA operon of Escherichia coli) was labeled with digoxigenin-II-dUTP by random priming and used to probe EcoRI fragments of L. monocytogenes chromosomal DNA on nylon filters. The method was successfully applied to the characterization of two sets of patient and food isolates of L. monocytogenes.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Geotechnical and geological engineering 15 (1997), S. 47-85 
    ISSN: 1573-1529
    Keywords: Numerical modelling ; mine design ; tabular stopes ; anisotropy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Summary This paper presents a rock mechanics design methodology applicable to steeply dipping orebodies typical of many underground hardrock mines. The first stage in the design process is the characterization of the rock mass using bothin situ and laboratory data. The effects of anisotropy on rock mass behaviour are discussed with reference to laboratory and field observations. The second stage involves the use of a number of selected numerical modelling techniques to investigate ground response in the near-field rock mass surrounding the mining excavations. This study shows that the use of several numerical methods in conjunction, allowing for the advantages of each method to be maximized, provides a more comprehensive analysis of the different facets of stope design. This approach differs from those in the literature which seek to compare the different numerical methods in order to select just one method best suited for a problem. The design methodology employed emphasizes the importance of developing an understanding of ground deformation mechanisms as opposed to predicting absolute behaviour.
    Type of Medium: Electronic Resource
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