ISSN:
1434-0879
Schlagwort(e):
Urinary bladder
;
Outlet obstruction
;
Denervation
;
Actin
;
Myosin
;
Desmin
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Medizin
Notizen:
Abstract The concentrations of the contractile proteins actin and myosin and the cytoskeletal protein desmin were determined in urinary bladders from normal rats, and from rats with bladder outlet obstruction or denervation. Ten days of obstruction or total denervation by bilateral removal of the pelvic ganglia resulted in an almost fourfold increase in bladder weight. Actin and myosin concentrations did not change significantly. The total amount of actin was 1624±235 μg in the control bladder. In the obstructed and denervated bladders it increased significantly to 6277±648 μg and 7671±835 μg, respectively. The desmin/actin ratio was 0.237±0.012 in the control bladders, and increased significantly to 0.369±0.015 in the obstructed and 0.343±0.022 in the denervated bladders. Partial denervation by removal of the pelvic ganglion on one side only increased bladder weight by 52%, but did not increase the desmin/actin ratio. The content of actin in such bladders increased by 82%. Both obstruction (which increases the functional load of the detrusor muscle cells) and denervation (which produces bladder paralysis) are known to induce hypertrophy of the detrusor smooth muscle cells. The study shows that the desmin/actin ratio and the total amount of contractile proteins increase in response to the hypertrophy as such, and not to the work performed by the smooth muscle cells, and that the nerves have no trophic influence on the growth response. Also, even a limited lesion of the bladder innervation is associated with growth and a net increase in the amount of contractile proteins.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00304076
Permalink
