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  • Multiple cell layers  (1)
  • PACS 14.40.Aq π, K and η mesons – 24.85.+p Quarks, gluons, and QCD in nuclei and nuclear processes  (1)
  • Plant microfilaments  (1)
Materialart
Erscheinungszeitraum
Schlagwörter
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Protoplasma 191 (1996), S. 215-219 
    ISSN: 1615-6102
    Schlagwort(e): Laser scanning confocal microscopy ; Multiple cell layers ; Plant microtubules ; Plant microfilaments ; Roots ; Tissue clearing
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A protocol was developed to observe plant microtubules and actin microfilaments in large tissue samples without physical sectioning. Rye (Secale cereale L. cv. Rymin) root tip pieces from two-day-old seedlings were fixed and processed for immunolabeling. Incubation times of 24–48 h were required to insure adequate penetration of fixatives, antibodies, and washing buffers. Clearing of the tissue with methyl salicylate reduced background auto-fluorescence that would otherwise interfere with the resolution of cytoskeletal structures. Microtubules or microfilaments in 5–7 cell layers were visualized using the optical-sectioning capability of laser scanning confocal microscopy (LSCM) and projected as three-dimensional images. The three-dimensional character of the cytoskeletal elements is retained when viewing stained cells of intact tissue. The net-like character of a microfilament array radiating out from a single point into the cytoplasm is maintained when the cells are stained in intact root tip pieces and imaging is accomplished in situ.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    The European physical journal 8 (2000), S. 283-289 
    ISSN: 1434-601X
    Schlagwort(e): PACS 14.40.Aq π, K and η mesons – 24.85.+p Quarks, gluons, and QCD in nuclei and nuclear processes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract: This paper is devoted to the evaluation of the pionic scalar density at finite temperature and baryonic density. We express the latter effect in terms of the nuclear response evaluated in the random phase approximation. We discuss the density and temperature evolution of the pionic density which governs the quark condensate evolution. Numerical evaluations are performed.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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