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  • 1
    Electronic Resource
    Electronic Resource
    PCR typing of human semen stains after SEM-EDX examination (1999)
    Springer
    International journal of legal medicine 112 (1999), S. 376-379 
    Details
    ISSN: 1437-1596
    Keywords: Key words Semen ; Scanning electron microscopy ; Electron dispersive x-ray microanalysis ; DNA typing ; PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract There is an increasing demand to use scanning electron microscopy in the forensic analysis of biological samples. Such analyses are routinely used for the investigation of blood stains, seminal stains, diatoms, residues on wounds and residues and trace elements in gunshot powder. The same samples are sometimes also required for identification via DNA analysis, e.g. blood stains, seminal stains or epidermal cells. The ionising radiation provokes damage to DNA and also to membrane and proteine structure. The question therefore arises whether the usual sequence of such an investigation, i.e. prior application of SEM and afterwards analysis of DNA, can affect the success rate of the DNA analysis. We have therefore experimentally exposed semen samples to a defined electron beam for different time intervals varying between 1 and 25 min and afterwards performed quantitative and qualitative DNA analysis. Our studies revealed that sample treatment with an electron beam does not interfere with subsequent DNA typing by various currently used forensic PCR systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004140050017
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    mtDNA as a tool for identification of human remains (1996)
    Springer
    International journal of legal medicine 109 (1996), S. 205-209 
    Details
    ISSN: 1437-1596
    Keywords: DNA typing ; Mitochondrial DNA ; Non-coding region ; D-loop region ; PCR ; Sequencing ; Identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract Two cases are presented in order to emphasize the importance of mitochondrial DNA in forensic medicine. The first case involved a charred body which could not be identified by morphological means because of severe destruction of all tissues. The parallel use of PCR methods using genomic DNA and sequencing of the mitochondrial d-loop region produced unequivocal and reproducible results. In the second case, various parts of a highly decomposed body were investigated. The application of standard PCR methods for genomic DNA proved unsuitable to answer the question whether the body parts belonged to the same body. However, when sequencing of mitochondrial DNA segments amplified from tissue and bone samples was performed, clearly interpretable results were obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01225519
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Location and frequency of polymorphic positions in the mtDNA control region of individuals from Germany (1998)
    Springer
    International journal of legal medicine 111 (1998), S. 67-77 
    Details
    ISSN: 1437-1596
    Keywords: Key words DNA typing ; Mitochondrial DNA ; Non-coding region ; D-loop region ; Hypervariable ; regions ; PCR ; Sequencing ; Population study
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract In order to identify polymorphic positions and to determine their frequency in the human mitochondrial D-loop containing region, the mitochondrial DNA (mtDNA) control region of 200 unrelated individuals from Germany were amplified and directly sequenced. Sequence comparison led to the identification of 190 mitochondrial lineages as defined by 202 variable positions. The most frequently occurring lineage comprised 5 individuals, whereas 186 types of D-loop sequences were observed in only one individual. Of the sequences studied 7% are not unique but show at least one counterpart with an identical haplotype. The majority (61%) of the control regions investigated showed between four and eight nucleotide positions deviating from the reference sequence. The maximum number of deviations observed in a single control region was 18. The majority of the variable positions in the D-loop region (88%) are located within three hypervariable regions. Sequence variations are caused by nucleotide substitutions, insertions or deletions. As compared to insertions and deletions, nucleotide substitutions make up the vast majority of the mutations (90%). We have predominantly found transitions (75%) and a significantly lower frequency of transversions (15%) whereas insertions (6%) as well as deletions (4%) are rather rare. Upon sequencing the mitochondrial control region from 200 German Caucasians the genetic diversity was estimated at 0.99. The probability of two randomly selected individuals from a population having identical mtDNA types is 0.6%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004140050117
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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