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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    International journal of legal medicine 113 (2000), S. 352-355 
    ISSN: 1437-1596
    Keywords: Keywords ABO genotyping ; PCR ; Restriction enzyme digestion ; Typing errors ; Blood stains
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract Genotypes of the ABO blood group system were investigated using a multiplex PCR and subsequent restriction enzyme digestion on experimental blood stains. Differences were found when typing blood between the PCR and serological methods and one blood sample, typed as B with the agglutination test was classified as AB using the method described here. The subsequent sequencing procedure revealed the genotype to be BB. Methodological causes for errors in typing which should be taken into consideration are discussed.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    International journal of legal medicine 110 (1997), S. 312-315 
    ISSN: 1437-1596
    Keywords: Key words Amelogenin ; Sex test ; Deletions ; PCR ; Alignment ; STRs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract When aligning the human amelogenin X (AmelX)-gene with the human amelogenin Y (AmelY)-gene, 19 regions of absolute homology ranging in size from 22 to 80 bp, 5 deletions located on AmelX from 1 to 6 bp and 5 deletions 1–183 bp long located on AmelY can be observed. The regions of absolute homology are used to design primer sets which span deletions of the X- and/ or Y-chromosome. The PCR products generated on the X- and Y-homologues differ in size. New regions of deletions and newly designed primers are described to facilitate the integration of the amelogenin sex test into multiplex PCR reactions.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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