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pMPY-ZAP: A reusable polymerase chain reaction-directed gene disruption cassette for Saccharomyces cerevisiae (1996)

Schneider, B. L. ; Steiner, B. ; Seufert, W. ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Yeast 12 (1996), S. 129-134

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ISSN: 0749-503X

Keywords: Saccharomyces cerevisiae ; gene disruption ; PCR-mediated disruption ; uraduction ; Life Sciences ; Life Sciences (general)

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology

Notes: Gene disruption is an important method for genetic analysis in Saccharomyces cerevisiae. We have designed a polymerase chain reaction-directed gene disruption cassette that allows rapid disruption of genes in S. cerevisiae without previously cloning them. In addition, this cassette allows recycling of URA3, generating gene disruptions without the permanent loss of the ura3 marker. An indefinite number of disruptions can therefore be made in the same strain.

Additional Material: 3 Ill.

Type of Medium: Electronic Resource

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