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  • 1
    Electronic Resource
    Electronic Resource
    Patch clamp analysis of excitatory synapses in mammalian spinal cord slices (1990)
    Springer
    Pflügers Archiv 417 (1990), S. 285-290 
    Details
    ISSN: 1432-2013
    Keywords: Spinal moto neurones ; Excitatory synaptic transmission ; NMDA and non-NMDA receptors ; Patch clamp ; Thin slice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Excitatory synaptic transmission to visually identified α-moto neurones was studied in thin slice preparations of the neonatal rat spinal cord. Excitatory postsynaptic currents (EPSCs) elicited by stimulation of intraspinal presynaptic fibres were recorded using the whole-cell patch clamp technique, following blockade of inhibitory transmission by bath application of strychnine and bicuculline. The EPSCs could be separated pharmacologically into N-methyl-d-aspartate- (NMDA) and non-NMDA-receptor-mediated components, where the contribution of the NMDA-mediated component was significant only at holding potentials more positive than −50 mV. Graded stimulation of intraspinal fibres showed that the NMDA- and the non-NMDA-mediated EPSCs were evoked by activation of presynaptic fibres with similar sensitivities to the stimulation intensity, suggesting that the same presynaptic fibres released the excitatory amino-acid (EAA) activating the two sub-sets of receptors. Studies of the amplitude fluctuations of EPSCs elicited by stimulation of a presumed single fibre revealed similar proportions of transmission failures and similar distributions of both the NMDA- and the non-NMDA-mediated components. These similarities suggest that the EAA transmitter activating the two sub-types of receptors is released from the same set of synaptic boutons and that the receptors are therefore post-synaptically co-localized. In addition the gamma aminobutyric acidB (GABAB) receptor agonist l-baclofen, which is known to decrease transmitter release, changed the amplitude distributions of non-NMDA- and NMDA-receptor-mediated EPSCs into unimodal distributions without affecting the amplitude of the presumed unitary event. The similarity between the transmitter release profiles of the two EAA components further supports the notion of postsynaptic receptor co-localization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00370994
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Voltage sensitivity of NMDA-receptor mediated postsynaptic currents (1990)
    Springer
    Experimental brain research 81 (1990), S. 209-212 
    Details
    ISSN: 1432-1106
    Keywords: NMDA ; Excitatory postsynaptic current ; Voltage sensitivity ; Patch clamp ; Thin hippocampal slice ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Patch-clamp techniques were used to record pharmacologically-isolated N-methyl-D-aspartate-mediated excitatory postsynaptic currents (NMDA-EPSCs) from dentate granule cells in thin rat hippocampal slices. Membrane voltage modulated these EPSCs in two ways. Firstly, depolarization from resting potential enhanced EPSC amplitudes, as expected for a voltage-dependent block by Mg2+ of synaptically activated NMDA receptor channels. Secondly, depolarization markedly prolonged the time course of decay of NMDA-EPSCs in normal and low extracellular Mg2+. Both mechanisms were complementary in establishing a strong dependence between membrane potential and the amount of charge, namely Ca2+, transferred through synaptically activated NMDA receptor channels, that presumably underlies induction of long-term potentiation in the hippocampus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00230117
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    A thin slice preparation for patch clamp recordings from neurones of the mammalian central nervous system (1989)
    Springer
    Pflügers Archiv 414 (1989), S. 600-612 
    Details
    ISSN: 1432-2013
    Keywords: Patch clamp ; Brain slice ; Central nervous system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract (1) A preparation is described which allows patch clamp recordings to be made on mammalian central nervous system (CNS) neurones in situ. (2) A vibrating tissue slicer was used to cut thin slices in which individual neurones could be identified visually. Localized cleaning of cell somata with physiological saline freed the cell membrane, allowing the formation of a high resistance seal between the membrane and the patch pipette. (3) The various configurations of the patch clamp technique were used to demonstrate recording of membrane potential, whole cell currents and single channel currents from neurones and isolated patches. (4) The patch clamp technique was used to record from neurones filled with fluorescent dyes. Staining was achieved by filling cells during recording or by previous retrograde labelling. (5) Thin slice cleaning and patch clamp techniques were shown to be applicable to the spinal cord and almost any brain region and to various species. These techniques are also applicable to animals of a wide variety of postnatal ages, from newborn to adult.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00580998
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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