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  • Nitrogen starvation  (1)
  • Pathogenicity  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Transcription of the avirulence gene Avr9 of the fungal tomato pathogen Cladosporium fulvum is regulated by a GATA-type transcription factor in Aspergillus nidulans (1999)
    Springer
    Molecular genetics and genomics 261 (1999), S. 653-659 
    Details
    ISSN: 1617-4623
    Keywords: Key words AREA ; niaD gene ; NIRA ; NIT2 ; Nitrogen starvation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The avirulence gene Avr9 of the fungal tomato pathogen Cladosporium fulvum is highly induced during infection of tomato plants. Expression of the Avr9 gene can also be induced in vitro when cells are grown on synthetic liquid medium containing little or no nitrogen. The Avr9 promoter contains six copies of the sequence TAGATA and six additional copies of the core sequence GATA within 0.4 kb upstream of the translation start site. In the filamentous fungi Aspergillus nidulans and Neurospora crassa, these promoter sequences have been identified as the binding sites for a wide-domain GATA-type regulator (AREA in A. nidulans and NIT2 in N. crassa) involved in nitrogen utilization. Quantification of GUS activity of A. nidulans transformants containing a single copy of the fully active Avr9 promoter-uidA (GUS) reporter gene fusion in different areA backgrounds, following starvation for nitrogen, showed that induction of the Avr9 promoter is regulated similarly in A. nidulans and C. fulvum. This suggests that AREA can regulate the Avr9 promoter and that C. fulvum contains an AREA-like regulator that can bind to these specific sequence motifs. Comparison of the induction profiles of Avr9 and niaD showed that Avr9 expression is independent of NIRA, as is niaD expression upon nitrogen starvation. Studies with Avr9 promoter-uidA fusions in which all or most of these sequences had been deleted, showed that Avr9 promoter activity is dependent on the presence of these specific cis-regulatory elements, suggesting that they do indeed function in transcriptional regulation of the Avr9 gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050008
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The Cf-ECP2 gene is linked to, but not part of, the Cf-4/Cf-9 cluster on the short arm of chromosome 1 in tomato (1999)
    Springer
    Molecular genetics and genomics 262 (1999), S. 839-845 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsLycopersicon esculentum ; Cladosporium fulvum ; Pathogenicity ; CAPS markers ; Hcr9
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A gene has been identified in tomato, which confers resistance to Cladosporium fulvum through recognition of the pathogenicity factor ECP2. Segregation analysis of F2 and F3 populations showed monogenic dominant inheritance, as for previously reported Cf resistances. The gene has been designated Cf-ECP2. Using several mapping populations, Cf-ECP2 was accurately mapped on chromosome 1, 7.7 cM proximal to TG236 and 6.0 cM distal to TG184. Although Cf-ECP2 is linked to Cf-4, it is not located in the Hcr9 cluster “Milky Way”. Therefore, Cf-ECP2 is the first functional Cf homologue on chromosome 1 that does not belong to this Hcr9 cluster. No recombination events between Cf-ECP2 and CT116 have been observed in three populations tested, representing 282 individuals. The low value for the physical distance per cM around CT116 reported previously and the high probability that Cf-ECP2 is also a member of a Hcr9 cluster will facilitate cloning of the locus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380051148
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    Paper (German National Licenses)
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