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  • Datura  (4)
  • Feedback regulation  (2)
  • Physics  (2)
  • 1
    Digitale Medien
    Digitale Medien
    Introduction of resistance to kanamycin into the protoplasts from a pantothenate-requiring auxotrophic cell line of Datura innoxia P. Mill. via direct gene transfer
    Amsterdam : Elsevier
    Plant Science 70 (1990), S. 105-114 
    Details
    ISSN: 0168-9452
    Schlagwort(e): Datura ; auxotrophy ; direct gene transfer ; kanamycin-resistance ; protoplasts ; universal hybridizer
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(90)90038-P
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  • 2
    Digitale Medien
    Digitale Medien
    Somatic hybrids derived from fusion of a universal hybridizer and a wild type line of Datura
    Amsterdam : Elsevier
    Plant Science 79 (1991), S. 111-118 
    Details
    ISSN: 0168-9452
    Schlagwort(e): Datura ; auxotrophy ; cell culture ; kanamycin resistance ; somatic hybrid ; universal hybridizer
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(91)90077-L
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  • 3
    Digitale Medien
    Digitale Medien
    A valine-resistant mutant of Arabidopsis thaliana displays an acetolactate synthase with altered feedback control (1994)
    Springer
    Planta 192 (1994), S. 249-255 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Acetolactate synthase ; Amino acid (branched chain) ; Arabidopsis ; Feedback regulation ; Mutant ; Valine resistance
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A valine-resistant mutant line, VAL-2, of Arabidopsis thaliana (L.) Heynh. was identified by screening M 2 populations of ethylmethane-sulfonate-mutagenized seeds. The resistance was found to be due to a single, dominant, nuclear gene mutation. Assay of acetolactate synthase (ALS) indicated that the valine resistance in this mutant is caused by decreased sensitivity of ALS to the branched-chain amino acids, valine, leucine and isoleucine. A two fold decrease in apparent K m value for pyruvate of the mutant ALS enzyme was detected compared with that of the wild type. The sensitivity of the ALS enzyme to sulfonylurea, imidazolinone and triazolopyrimidine herbicides was not altered in the mutant. At the plant growth level the mutant was also resistant to valine plus leucine, but was sensitive to leucine or isoleucine alone. The mutant gene, var1, maps, or is very closely linked, to CSR1, the gene encoding acetolactate synthase in Arabidopsis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00194459
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  • 4
    Digitale Medien
    Digitale Medien
    Transplantation of isolated nuclei into plant protoplasts (1986)
    Springer
    Planta 168 (1986), S. 29-35 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Auxotrophy ; Datura ; DNA transfer ; Nuclear transplantation ; Protoplast ; Prototrophy ; Vicia (nuclear transplantation)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The uptake of isolated nuclei from Vicia hajastana Grossh. cells into protoplasts of an auxotrophic cell line of Datura innoxia P. Mill. was induced under the influence of polyethylene glycol and Ca2+ at pH 6.8. The frequency of nuclear uptake varied from 0.8 to 2.3% and that of the recovery of prototrophic clones from 10-5 to 6·10-4. The prototrophic nuclear fusion products following nuclear uptake could be rescued by initial culture of the protoplasts in non-selective conditions and by the subsequent use of feeder cell layers to support the growth of surviving colonies on a selective medium. The presence of Vicia genomic DNA in some prototrophic clones was confirmed by dot-blot hybridization using Datura and Vicia DNA probes. In certain transformed clones, the recovery of prototrophy was accompanied by the restoration of morphogenetic potential. Welldeveloped shoots typical of wild-type Datura could be regenerated employing an appropriate regeneration medium.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00407005
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  • 5
    Digitale Medien
    Digitale Medien
    A transient duplication of the acetolactate synthase gene in a cell culture of Datura innoxia (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 417-422 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Gene amplification ; Datura ; Sulfonylurea resistance ; Plant cell culture
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A 2.0 kb fragment of the yeast ILV2 gene, which codes for the target enzyme acetolactate synthase (ALS) of the herbicide chlorsulfuron, was shown to hybridize to the nuclear DNA of a haploid cell culture of Datura innoxia P. Mill. Nuclear DNA of a chlorsulfuron resistant line of D. innoxia, CSR6, gave a prominent 2.65 kb band when cleaved by either EcoRI or HindIII. The 2.65 kb band has been shown to hybridize with the yeast ILV2 probe. A herbicide resistant line descended from CSR6 by continuous culture resulted in the loss of the 2.65 kb restriction fragment. These observations suggest that CSR6 resulted from a large tandem duplication of the ALS gene and that a point mutation for herbicide resistance in an ALS gene repeat unit of the duplication was selected during subsequent growth of the resistant line.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00289814
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  • 6
    Digitale Medien
    Digitale Medien
    A valine-resistant mutant ofArabidopsis thaliana displays an acetolactate synthase with altered feedback control (1994)
    Springer
    Planta 192 (1994), S. 249-255 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Acetolactate synthase ; Amino acid (branched chain) ; Arabidopsis ; Feedback regulation ; Mutant ; Valine resistance
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A valine-resistant mutant line, VAL-2, ofArabidopsis thaliana (L.) Heynh. was identified by screening M 2 populations of ethylmethane-sulfonate-mutagenized seeds. The resistance was found to be due to a single, dominant, nuclear gene mutation. Assay of acetolactate synthase (ALS) indicated that the valine resistance in this mutant is caused by decreased sensitivity of ALS to the branched-chain amino acids, valine, leucine andisoleucine. A two fold decrease in apparentK m value for pyruvate of the mutant ALS enzyme was detected compared with that of the wild type. The sensitivity of the ALS enzyme to sulfonylurea, imidazolinone and triazolopyrimidine herbicides was not altered in the mutant. At the plant growth level the mutant was also resistant to valine plus leucine, but was sensitive to leucine orisoleucine alone. The mutant gene,var1, maps, or is very closely linked, toCSR1, the gene encoding acetolactate synthase inArabidopsis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01089041
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  • 7
    Digitale Medien
    Digitale Medien
    Solute absorption into molten polystyrene (1972)
    New York : Wiley-Blackwell
    Journal of Polymer Science Part A-1: Polymer Chemistry 10 (1972), S. 689-699 
    Details
    ISSN: 0449-296X
    Schlagwort(e): Physics ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: The equilibrium volatilities at near infinite dilution of various solutes absorbed in molten polystyrene have been determined by a gas chromatographic technique. This method is much more rapid, although, with the present apparatus, probably less accurate than conventional static techniques. The primary parameters obtained from measurements of retention volumes are the Henry's law constants, from which are derived the weight and volume fraction activity coefficients, the Flory-Huggins interaction parameters, and the heats of dilution and solution.Of the solutes investigated, 2-butanone (MEK) was the least, and benzene the most compatible (highest and lowest volume fraction activity coefficients, respectively) with molten polystyrene. A small, but definite, variation of the activity coefficients with polystyrene molecular weight was observed.
    Zusätzliches Material: 6 Tab.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/pol.1972.150100306
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  • 8
    Digitale Medien
    Digitale Medien
    Inorganic coordination polymers. XII. Chromium(III) tris(phosphinates) (1973)
    New York : Wiley-Blackwell
    Journal of Polymer Science: Polymer Chemistry Edition 11 (1973), S. 2691-2701 
    Details
    ISSN: 0360-6376
    Schlagwort(e): Physics ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: Cohesive films of a series of chromium(III) tris(phosphinates)[Cr(OPRR′O)(OPR″-R‴O)(OPR*R**O)]y in which the side groups on the phosphorus are alkyl, phenyl, and/or hydrogen groups have been prepared by reaction casting or by hot pressing. The preparative reaction appears to involve conversion of an aquahydroxo or hydroxo chromium(III) bis(phosphinate) to an intermediate hydroxo bis(phosphinate) containing coordinated phosphinic acid followed by an intrachain reaction leading to the tris(phosphinate). Tensile strengths of the chromium(III) tris(phosphinates) range from 100 to 5600 psi, elongations from less than 1 to 100%. Thermogravimetric data indicate that major decomposition occurs at temperatures from 200 to 435°C in air and from 410 to 510°C in nitrogen. Infrared spectra and physical properties are interpreted to support a structure for the chromium(III) tris(phosphinates) which is based on linear, triple-bridged chains. The presence of octyl or other long alkyl side groups on the phosphorus improves the physical properties, presumably by internal plasticization, but leads to decreased thermal stability.
    Zusätzliches Material: 2 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/pol.1973.170111021
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