ISSN:
1617-4623
Keywords:
Plasmid replication
;
Incompatibility
;
Helix-turn-helix motifs
;
Superinfection immunity
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary We report here the existence and initial characterization of a genetic locus (imp) that inhibits maintenance of SLP1-derived plasmids as extrachromosomal replicons in a manner distinct from normal incompatibility between autonomous SLP1 replicons. Thetrans-actingimp function has been localized to a 1.8 kbEco47III restriction fragment present on integrated SLP1 elements. At least part of this DNA segment is absent from SLP1-derived plasmids. DNA sequence analysis of theimp region indicates that it contains three overlapping open reading frames (ORFs) that may constitute a polycistronic operon. The effects of insertions within theimp region indicate that uninterrupted transcription through all three ORFs is necessary forimp activity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02464900
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