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Characterization of the genome of a vaccine strain of canine adenovirus type 1 (1988)

Liu, Y. -C. ; Abouhaidar, M. G. ; Sira, S. ; [et al.]

Springer

Virus genes 2 (1988), S. 69-81

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ISSN: 1572-994X

Keywords: canine adenovirus type 1 vaccine ; restriction endonuclease analysis ; genomic physical mapping ; deletion mutants ; dog kidney cells ; virulence

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract Restriction endonuclease cleavage maps have been constructed for the genome of a canine adenovirus type 1 (CAV-1) vaccine strain (CLL; Connaught Laboratories, Ltd., Willowdale, Ontario). Restriction enzyme analyses were also carried out on CAV-1 (CLL) genomes isolated from viral stocks over 8 serial passages in a dog kidney cell line (DK 6722). The right hand 20% of the genome became more heterogeneous in size with increasing passage in DK 6722 cells due to deletions up to 3–4 kb, whereas the left terminal region was stable throughout these passages. A comparative study of CAV-1(CLL) and a virulent strain of CAV-1, Glaxo, revealed that the genome of CAV-1(CLL) was the shorter, by about 480 bp, within the region covering 0.83–0.91 map units. By virtue of its location within the genome and its dispensable nature for viral growth, this region would appear to encompass a genetic sequence corresponding to the E3 region of human adenoviruses. In terms of viral attenuation, the possible importance of the observed differences between CAV-1(CLL) and CAV-1(Glaxo) is discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00569737

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Effect of impurities on emulsion polymerization: Case II kinetics (1988)

Huo, B. P. ; Campbell, J. D. ; Penlidis, A. ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Journal of Applied Polymer Science 35 (1988), S. 2009-2021

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ISSN: 0021-8995

Keywords: Chemistry ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics

Notes: The effects of water-soluble and monomer-soluble impurities on the kinetics of emulsion polymerization of monomers following Case II kinetics (e.g., styrene) are investigated. Experimental studies reveal that impurities can have an appreciable effect on both polymer particle nucleation and growth. These effects are shown to be well predicted by a mathematical model.

Additional Material: 9 Ill.