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  • Polymer and Materials Science  (8)
  • Datura  (4)
  • Feedback regulation  (2)
Materialart
Erscheinungszeitraum
Schlagwörter
  • 1
    ISSN: 0168-9452
    Schlagwort(e): Datura ; auxotrophy ; direct gene transfer ; kanamycin-resistance ; protoplasts ; universal hybridizer
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Amsterdam : Elsevier
    Plant Science 79 (1991), S. 111-118 
    ISSN: 0168-9452
    Schlagwort(e): Datura ; auxotrophy ; cell culture ; kanamycin resistance ; somatic hybrid ; universal hybridizer
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Planta 168 (1986), S. 29-35 
    ISSN: 1432-2048
    Schlagwort(e): Auxotrophy ; Datura ; DNA transfer ; Nuclear transplantation ; Protoplast ; Prototrophy ; Vicia (nuclear transplantation)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The uptake of isolated nuclei from Vicia hajastana Grossh. cells into protoplasts of an auxotrophic cell line of Datura innoxia P. Mill. was induced under the influence of polyethylene glycol and Ca2+ at pH 6.8. The frequency of nuclear uptake varied from 0.8 to 2.3% and that of the recovery of prototrophic clones from 10-5 to 6·10-4. The prototrophic nuclear fusion products following nuclear uptake could be rescued by initial culture of the protoplasts in non-selective conditions and by the subsequent use of feeder cell layers to support the growth of surviving colonies on a selective medium. The presence of Vicia genomic DNA in some prototrophic clones was confirmed by dot-blot hybridization using Datura and Vicia DNA probes. In certain transformed clones, the recovery of prototrophy was accompanied by the restoration of morphogenetic potential. Welldeveloped shoots typical of wild-type Datura could be regenerated employing an appropriate regeneration medium.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Planta 192 (1994), S. 249-255 
    ISSN: 1432-2048
    Schlagwort(e): Acetolactate synthase ; Amino acid (branched chain) ; Arabidopsis ; Feedback regulation ; Mutant ; Valine resistance
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A valine-resistant mutant line, VAL-2, ofArabidopsis thaliana (L.) Heynh. was identified by screening M 2 populations of ethylmethane-sulfonate-mutagenized seeds. The resistance was found to be due to a single, dominant, nuclear gene mutation. Assay of acetolactate synthase (ALS) indicated that the valine resistance in this mutant is caused by decreased sensitivity of ALS to the branched-chain amino acids, valine, leucine andisoleucine. A two fold decrease in apparentK m value for pyruvate of the mutant ALS enzyme was detected compared with that of the wild type. The sensitivity of the ALS enzyme to sulfonylurea, imidazolinone and triazolopyrimidine herbicides was not altered in the mutant. At the plant growth level the mutant was also resistant to valine plus leucine, but was sensitive to leucine orisoleucine alone. The mutant gene,var1, maps, or is very closely linked, toCSR1, the gene encoding acetolactate synthase inArabidopsis.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Planta 192 (1994), S. 249-255 
    ISSN: 1432-2048
    Schlagwort(e): Acetolactate synthase ; Amino acid (branched chain) ; Arabidopsis ; Feedback regulation ; Mutant ; Valine resistance
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A valine-resistant mutant line, VAL-2, of Arabidopsis thaliana (L.) Heynh. was identified by screening M 2 populations of ethylmethane-sulfonate-mutagenized seeds. The resistance was found to be due to a single, dominant, nuclear gene mutation. Assay of acetolactate synthase (ALS) indicated that the valine resistance in this mutant is caused by decreased sensitivity of ALS to the branched-chain amino acids, valine, leucine and isoleucine. A two fold decrease in apparent K m value for pyruvate of the mutant ALS enzyme was detected compared with that of the wild type. The sensitivity of the ALS enzyme to sulfonylurea, imidazolinone and triazolopyrimidine herbicides was not altered in the mutant. At the plant growth level the mutant was also resistant to valine plus leucine, but was sensitive to leucine or isoleucine alone. The mutant gene, var1, maps, or is very closely linked, to CSR1, the gene encoding acetolactate synthase in Arabidopsis.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 74 (1987), S. 417-422 
    ISSN: 1432-2242
    Schlagwort(e): Gene amplification ; Datura ; Sulfonylurea resistance ; Plant cell culture
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A 2.0 kb fragment of the yeast ILV2 gene, which codes for the target enzyme acetolactate synthase (ALS) of the herbicide chlorsulfuron, was shown to hybridize to the nuclear DNA of a haploid cell culture of Datura innoxia P. Mill. Nuclear DNA of a chlorsulfuron resistant line of D. innoxia, CSR6, gave a prominent 2.65 kb band when cleaved by either EcoRI or HindIII. The 2.65 kb band has been shown to hybridize with the yeast ILV2 probe. A herbicide resistant line descended from CSR6 by continuous culture resulted in the loss of the 2.65 kb restriction fragment. These observations suggest that CSR6 resulted from a large tandem duplication of the ALS gene and that a point mutation for herbicide resistance in an ALS gene repeat unit of the duplication was selected during subsequent growth of the resistant line.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 7
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 35 (1988), S. 787-796 
    ISSN: 0021-8995
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Maschinenbau , Physik
    Notizen: The Young's moduli of samples of poly(vinyl chloride) containing either no plasticizer or 5 pph of dioctyl sebacate were drawn uniaxially at 80°C to draw ratios, λ, in the range 1-3.3. The moduli, determined by a three-point bending method were found to increase monotonically with λ to a value of 6.3 GPa for the nonplasticized samples and to reach a maximum value of about 4.5 GPa for the plasticized samples above λ = 2.3, which showed voiding. The lower moduli of the plasticized samples for a given degree of orientation, as assessed from birefringence measurements, can be attributed to the diluent effect of the plasticizer on the load-bearing chains. The Raman measurements suggest that the crystallites probably orient rather like rigid rods in an affinely deforming matrix, with some relaxation.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 45 (1992), S. 947-956 
    ISSN: 0021-8995
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Maschinenbau , Physik
    Notizen: The peel strength and the color of the copper foil peeled at 90 degrees from five different polyimide films were studied. The interfacial surfaces of copper foil and polyimide were examined by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and energy dispersion analysis by X-ray (EDAX). There is a correlation between peel strength, and the color of the interfacial side copper caused by oxygen diffusion. Study of the imidization process carried out in vacuum indicates that the geometric arrangements of the atoms of polyimide also play a very important role in peel strength.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 9
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 29 (1984), S. 3863-3872 
    ISSN: 0021-8995
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Maschinenbau , Physik
    Notizen: A rapid, reproducible method for investigating the molecular mas distribution of urea formaldehyde resins by size exclusion chromatography has been developed. By using concentrated lithium chloride solution to prepare the sample, materials of high viscosity and high molecular mass can be easily dissolved. Chromatography in dimethyl formamide containing lithium chloride eliminates hydrogen bonding and ensures that realistic values for molecular mass averages are obtained.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 10
    Digitale Medien
    Digitale Medien
    New York : Wiley-Blackwell
    Journal of Polymer Science: Polymer Chemistry Edition 11 (1973), S. 2691-2701 
    ISSN: 0360-6376
    Schlagwort(e): Physics ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: Cohesive films of a series of chromium(III) tris(phosphinates)[Cr(OPRR′O)(OPR″-R‴O)(OPR*R**O)]y in which the side groups on the phosphorus are alkyl, phenyl, and/or hydrogen groups have been prepared by reaction casting or by hot pressing. The preparative reaction appears to involve conversion of an aquahydroxo or hydroxo chromium(III) bis(phosphinate) to an intermediate hydroxo bis(phosphinate) containing coordinated phosphinic acid followed by an intrachain reaction leading to the tris(phosphinate). Tensile strengths of the chromium(III) tris(phosphinates) range from 100 to 5600 psi, elongations from less than 1 to 100%. Thermogravimetric data indicate that major decomposition occurs at temperatures from 200 to 435°C in air and from 410 to 510°C in nitrogen. Infrared spectra and physical properties are interpreted to support a structure for the chromium(III) tris(phosphinates) which is based on linear, triple-bridged chains. The presence of octyl or other long alkyl side groups on the phosphorus improves the physical properties, presumably by internal plasticization, but leads to decreased thermal stability.
    Zusätzliches Material: 2 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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