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Digitale Medien

Identification of nucleotides critical for activity of the Pseudomonas putida catBC promoter (1989)

Aldrich, Teri L. ; Rothmel, Randi Kubrick ; Chakrabarty, A. M.

Springer

Molecular genetics and genomics 218 (1989), S. 266-271

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ISSN: 1617-4623

Schlagwort(e): Benzoate degradation ; Positive regulation ; Site-specific mutagenesis ; Primer extension ; Catechol

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Summary Pseudomonas putida utilizes the catBC operon, which encodes cis,cis-muconate lactonizing enzyme I (MLEI; EC 5.5.1.1) and muconolactone isomerase (MI; EC 5.3.3.4), for growth on benzoate as a sole carbon source. This operon is positively regulated, and the promoter is located 64 bp upstream of the catB translational start site. Using site-specific mutagenesis, we identified nucleotides that influenced the induction of this promoter. Promoter activity was monitored with the promoter probe vector pKT240. Transcription of mRNA from mutant promoters was determined by primer extension mapping. Comparison of the initiation start site of mutant promoters with that of the wild-type promoter identified a single functional promoter.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00331277

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Digitale Medien

Molecular basis of biodegradation of chloroaromatic compounds (1989)

Sangodkar, U. M. X. ; Aldrich, T. L. ; Haugland, R. A. ; [weitere]

Berlin : Wiley-Blackwell

Acta Biotechnologica 9 (1989), S. 301-316

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Details

ISSN: 0138-4988

Schlagwort(e): Life Sciences ; Life Sciences (general)

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Chlorinated aromatic hydrocarbons are widely used in industry and agriculture, and comprise the bulk of environmental pollutants. Although simple aromatic compounds are biodegradable by a variety of degradative pathways, their halogenated counterparts are more resistant to bacterial attack and often necessitate evolution of novel pathways. An understanding of such evolutionary processes is essential for developing genetically improved strains capable of mineralizing highly chlorinated compounds. This article provides an overview of the genetic aspects of dissimilation of chloroaromatic compounds and discusses the potential of gene manipulation to promote enhanced evolution of the degradative pathways.

Zusätzliches Material: 5 Ill.