ISSN:
1617-4623
Keywords:
Benzoate degradation
;
Positive regulation
;
Site-specific mutagenesis
;
Primer extension
;
Catechol
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Pseudomonas putida utilizes the catBC operon, which encodes cis,cis-muconate lactonizing enzyme I (MLEI; EC 5.5.1.1) and muconolactone isomerase (MI; EC 5.3.3.4), for growth on benzoate as a sole carbon source. This operon is positively regulated, and the promoter is located 64 bp upstream of the catB translational start site. Using site-specific mutagenesis, we identified nucleotides that influenced the induction of this promoter. Promoter activity was monitored with the promoter probe vector pKT240. Transcription of mRNA from mutant promoters was determined by primer extension mapping. Comparison of the initiation start site of mutant promoters with that of the wild-type promoter identified a single functional promoter.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00331277
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