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  • Key words: Annexin V — Chondrocytes — Nitric oxide — Apoptosis — MAP kinase  (1)
  • Protoplast  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Brassinolide affects the rate of cell division in isolated leaf protoplasts of Petunia hybrida (1998)
    Springer
    Plant cell reports 17 (1998), S. 921-924 
    Details
    ISSN: 1432-203X
    Keywords: Key words Brassinosteroid ; Brassinolide ; Protoplast ; Cell division
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Brassinosteroids are known to promote cell elongation in a wide range of plant species but their effect on cell division has not been as extensively studied. We examined the effect of brassinolide on the kinetics and final division frequencies of regenerating leaf mesophyll protoplasts of Petunia hybrida Vilm v. Comanche. Under optimal auxin and cytokinin conditions, 10–100 nM brassinolide accelerated the time of first cell division by 12 h but had little effect on the final division frequencies after 72–120 h of culture. One micromolar brassinolide showed the same acceleration of first cell division but inhibited the final division frequency by approximately 20%. Under sub-optimal auxin conditions, 10–100 nM brassinolide both accelerated the time of first cell division and dramatically increased the 72- to 120-h final division frequencies. Isolated protoplasts may provide a useful model system to investigate the molecular mechanisms of brassinosteroid action on cell proliferation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050510
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of hydrogen peroxide on the metabolism of articular chondrocytes (1999)
    Springer
    Inflammation research 48 (1999), S. 399-403 
    Details
    ISSN: 1420-908X
    Keywords: Key words: Annexin V — Chondrocytes — Nitric oxide — Apoptosis — MAP kinase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Objective: To examine the effect of hydrogen peroxide on chondrocyte metabolism.¶Materials and Methods: Bovine articular chondrocytes were used. Proteoglycan (PG) synthesis was measured with [35S] sulfate incorporation. For detection of apoptosis, the TdT-mediated dUTP-biotin nick end labeling (TUNEL) and annexin V assay were used. Extracellular-regulated protein kinase (ERK) activity was measured using a mitogen-activated protein kinase assay system.¶Results: Addition of hydrogen peroxide resulted in the inhibition of PG synthesis, apoptosis, and enhanced ERK activity.¶Conclusion: Hydrogen peroxide plays an important role in regulating the metabolism of chondrocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s000110050478
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    Paper (German National Licenses)
    Fulltext
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