ISSN:
1432-072X
Keywords:
Aminolaevulinic acid
;
Aminotransferase
;
Chlorophyll
;
Cyanobacterium
;
Gabaculin
;
Glutamate semialdehyde
;
Pyridoxal
;
Pyridoxamine
;
Synechococcus
;
Tetrapyrrole
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Glutamate 1-semialdehyde aminotransferase has been separated from metabolically related activities by gel filtration and affinity chromatography. The enzyme was inhibited by gabaculin, 4-amino 5-fluoropentanoic acid and pyridoxal 5-phosphate and stimulated by pyridoxamine 5-phosphate. The activity of enzyme recovered by elution after electrophoresis in non-denaturing polyacrylamide gels was wholly dependent on pyridoxamine 5-phosphate. A mechanism for the enzyme-catalysed reaction based on these observations is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00249178
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