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Avian spinal cord chimeras. Further studies on the neurological syndrome affecting the chimeras after birth

Kinutani, M. ; Desaki, J. ; Takashima, Y. ; [et al.]

Amsterdam : Elsevier

Cell Differentiation and Development 26 (1989), S. 145-162

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ISSN: 0922-3371

Keywords: Demyelinating disease ; Neuromuscular junction ; Quail -〉 chick spinal cord chimeras ; Tolerance

Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Topics: Biology

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1016/0922-3371(89)90016-6

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Nitrile hydratase from a thermophilic Bacillus smithii (1998)

Takashima, Y ; Yamaga, Y ; Mitsuda, S

Springer

Journal of industrial microbiology and biotechnology 20 (1998), S. 220-226

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ISSN: 1476-5535

Keywords: Keywords: nitrile hydratase; thermophile; Bacillus smithii

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: A novel thermophilic Bacillus smithii strain SC-J05-1, isolated from a hot spring, had the ability of hydrating nitrile to form amide. The nitrile hydratase was purified to homogeneity from the microbial cells of SC-J05-1 and was characterized. The enzyme was a 130-kDa protein composed of two different subunits (25.3 kDa and 26.8 kDa) and contained cobalt ions. This enzyme had the optimal temperature of 40°C and was stable up to 50°C. The optimal pH was in the alkaline region higher than pH 10.