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  • 1985-1989  (2)
  • C-banding  (1)
  • Relic DNA  (1)
  • 1
    ISSN: 1432-2242
    Keywords: Repetitive DNA ; Wheat ; Rye ; Relic DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The construction and molecular analysis of four recombinant clones — pTa1, pTa2, pTa7, and pTa8 — is described. The four clones contain different highly repeated sequences of genomic DNA from Triticum aestivum variety ‘Chinese Spring’. The wheat specificity has been determined by colony and dot blot hybridization in comparison with total rye DNA (Secale cereale variety ‘Petka’). The four clones with a variable degree of specificity were compared by sequence analysis after the recloning of wheat DNA inserts into M13 mp8. Within the sequencing data a tendency can be observed that those repeated sequences which show the highest degree of species specificity contain a significantly increased amount of GC residues.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 72 (1986), S. 721-734 
    ISSN: 1432-2242
    Keywords: Nuclear cytology ; C-banding ; Chromosome nomenclature ; Gene localization ; Trisomies ; Rye
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Progress in rye karyology is reviewed with respect to chromosome structure, recognition and chromosome nomenclature. Considerable contributions have been brought about by molecular techniques which have even revealed nucleotide sequences of some of the ribosomal DNA. DNA sequence organization correlates with the distribution of major Giemsa C-band regions as well as with N-bands and the binding sites of fluorescent dyes. The several banding patterns permit the classification of rye chromosomes. The increased data and widespread application of banding analysis require a consistent system of chromosome and/or band designation. Therefore, a standard band nomenclature is proposed with reference to the recommendations of the “Paris Conference on Standardization in Human Cytogenetics”. In addition, advances in genetics are summarized and discussed. Based on the original accepted standard karyogram and banding patterns of the rye chromosomes, meanwhile, 120 genes determining several characters have been associated with individual chromosomes and/or chromosome arms, including linkage studies for about 19 arrangements. Most results were obtained using wheat-rye addition lines as well as test crosses with defined translocations. Moreover, genetical studies based on appropriate trisomic and telotrisomic material resulted in the localization of 19 genes, including their linkage relationships.
    Type of Medium: Electronic Resource
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