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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 222 (1990), S. 337-344 
    ISSN: 1617-4623
    Keywords: Plasmid transfer ; Repressor ; Transcription control ; lac genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary ThekorA andkorB loci ofStreptomyces lividans plasmid pIJ101 have previously been shown to control expression of the pIJ101tra (formerlykilA) andkilB genes at the transcriptional level. We show here that mutations in translational open reading frames (ORFs) that map within the kor loci abolish repression of theS. lividans lac gene directed by thetra andkilB promoters. Introduction of thekorA andkorB ORFs intoEscherichia coli maxicells under control of anE. coli promoter gave rise to 31 kDa and 10 kDa proteins that correspond in size to the products expected from the sequences of the respective ORFs; these proteins controlled transcription from the pIJ101tra andkilB promoters in theE. coli host. Mutations that affected the KorA or KorB phenotype altered the structure of, or eliminated, the protein products of thekorA andkorB ORFs, further demonstrating that these ORFs encode the functional repressors of the pIJl01kil/kor gene system.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1617-4623
    Keywords: Plasmid replication ; Incompatibility ; Helix-turn-helix motifs ; Superinfection immunity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We report here the existence and initial characterization of a genetic locus (imp) that inhibits maintenance of SLP1-derived plasmids as extrachromosomal replicons in a manner distinct from normal incompatibility between autonomous SLP1 replicons. Thetrans-actingimp function has been localized to a 1.8 kbEco47III restriction fragment present on integrated SLP1 elements. At least part of this DNA segment is absent from SLP1-derived plasmids. DNA sequence analysis of theimp region indicates that it contains three overlapping open reading frames (ORFs) that may constitute a polycistronic operon. The effects of insertions within theimp region indicate that uninterrupted transcription through all three ORFs is necessary forimp activity.
    Type of Medium: Electronic Resource
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