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  • Cell & Developmental Biology  (38)
  • SOLID Retrieval/Processing System  (6)
  • Agaricus  (5)
  • 1
    ISSN: 1432-0983
    Schlagwort(e): Agaricus ; MtDNA ; Restriction map ; Inverted repeat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Mitochondrial (mt) DNA from the commercial mushroom Agaricus brunnescens Peck [= A. bisporus (Lange) Imbach] was purified by cesium chloride/bisbenzimide gradient centrifugation. A physical map of the mtDNA fragments produced by BamHI, EcoRl, and PvuII digestion was generated by filter hybridizations with radiolabelled BamHI mtDNA probes. The A. brunnescens mtDNA was a circular molecule 136 kilo-basepairs (kbp) in length and contained an inverted repeat between 4.6 and 9.2 kbp in size. Orientational isomers of the mitochondrial genome were not detected. The positions of six genes were located on the A. brunnescens mtDNA map by heterologous hybridization. No coding function has yet been ascribed to the inverted repeat. The large rRNA gene was located on the smaller single copy region. The genes for cytochrome b, cytochrome oxidase (subunit III), ATPase (subunits 8 and 6) and the small rRNA were located on different regions of the larger single copy region.
    Materialart: Digitale Medien
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  • 2
    ISSN: 1432-0983
    Schlagwort(e): Agaricus ; Mitochondria ; Plasmid ; RNA polymerase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Agaricus bisporus, the cultivated mushroom, contains a mitochondrial fragment (50H) which was previously demonstrated by Southern hybridization to have sequence similarity to an internal region of pEM, a linear mitochondrial plasmid of Agaricus bitorquis. The nucleotide sequence of 50H was determined and compared to the sequence of the corresponding pEM fragment. The region of sequence homology on pEM is contained within an open reading frame (ORF) that may encode an RNA polymerase, but 50H is neither an intact nor a complete copy of the ORF. pEM also contains an ORF with characteristics of genes for virus-encoded DNA polymerases. pEM appears to be very similar to other linear mitochondrial plasmids (in fungi and higher plants) reported to contain ORFs that may encode the same types of polymerases. The potential functionality of the pEM sequence suggests that it has diverged less than the mitochondrial fragment from a common ancestor.
    Materialart: Digitale Medien
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Current genetics 29 (1996), S. 370-376 
    ISSN: 1432-0983
    Schlagwort(e): Plasmid ; RNA polymerase ; Mitochondrion ; Agaricus
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A linear mitochondrial plasmid, pEM, found in certain isolates of the basidiomyceteAgaricus bitorquis potentially encodes virus-like DNA and RNA polymerases. Mitochondrial DNA fromAgaricus bisporus that hybridizes to an internal region of pEM contains a fragmented and potentially non-functional version of the carboxy terminal end of the plasmid RNA polymerase. In this study, we present the sequence of the corresponding region of mitochondrial DNA fromA. bitorquis. This sequence contained the same region of the plasmid RNA polymerase gene as was reported for the mitochondrial DNA ofA. bisporus, and the level of similarity between theA. bisporus andA. bitorquis mitochondrial sequences was much higher than the level of similarity between either mitochondrial sequence and the plasmid. We propose that this plasmid RNA polymerase-like sequence was present in theAgaricus mitochondrial genome before the divergence ofA. bisporus andA. bitorquis, and thus is unlikely to be a recent derivative of the plasmid pEM.
    Materialart: Digitale Medien
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Current genetics 8 (1984), S. 615-619 
    ISSN: 1432-0983
    Schlagwort(e): Agaricus ; Plasmid-like DNAs ; Mitochondrial DNA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Two unique plasmid-like DNA components were localized in isolated mitochondria of the commercially important mushroom genus Agaricus: pEM (7.35 ± 0.15 kilobases) and pMPJ (3.65 ± 0.15 kilobases). These DNA moieties were linear; pEM possessed regions of terminal inverted repeated sequences. No homology was detected between pEM or pMPJ DNA and the nuclear or mitochondrial genomes. No homology existed between pEM and pMPJ. This suggests independent replication of pEM and pMPJ. Restriction endonuclease digests indicated that pEM consisted of two components (pEM1 and pEM2) with uniquely different restriction sites and copy number.
    Materialart: Digitale Medien
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  • 5
    ISSN: 1432-0983
    Schlagwort(e): Agaricus ; Mitochondrial DNA ; Restriction pattern polymorphism ; Restriction endonuclease analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Mitochondrial DNAs were isolated from four cultivated strains of the commercial two-spored mushroom Agaricus brunnescens (bisporus) and from ten isolates of the four spored mushroom Agaricus bitorquis. Digestion of the fungal mitochondrial DNA with restriction endonucleases yielded numerous fragments. Summation of the fragment sizes gave a mitochondrial DNA size of 98.3 ± 2.4 kilobases (kb) (64.9 x 106 daltons) for A. brunnescens. The size of the mitochondrial DNA ranged from 148.5 ± 10.8 kb (98.0 x 106 daltons) to 176.3 ± 12.0 kb (116.4 x 106 daltons) for A. bitorquis. The restriction patterns, produced by a variety of endonucleases, were identical for all four isolates of A. brunnescens. The ten isolates of A. bitorquis demonstrated extensive restriction pattern heterogeneity and have been tentatively assigned into four groups. Approximately 60% of the A. bitorquis mitochondrial DNA restriction fragments show sequence homology with A. brunnescens mitochondrial DNA based on DNA — DNA hybridizations.
    Materialart: Digitale Medien
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  • 6
    ISSN: 1573-8787
    Schlagwort(e): Transparent Query Language ; Mathematically Complete Language ; Philosophically Closed Language ; SOLID Retrieval/Processing System
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Informatik
    Notizen: Abstract This part, PART IIB [2], of the document “HIGH-SPEED TOOLS FOR GLOBAL INFORMATION MANAGEMENT. II. Specifications and Uses of the Transparent Query Language (TQL)” [1–6] contains the specifications for the operations that provide the arithmetic capabilities for Transparent Query Language. PART IIB references PART IIA [1] and PART IIC [3]. Concise definitions of Transparent Query Language terms, Conclusions and Acknowledgments are given in PART IIF [6].
    Materialart: Digitale Medien
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  • 7
    ISSN: 1573-8787
    Schlagwort(e): Transparent Query Language ; Mathematically Complete Language ; Philosophically Closed Language ; SOLID Retrieval/Processing System
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Informatik
    Notizen: Abstract This part, PART IIC [3], of the document “HIGH-SPEED TOOLS FOR GLOBAL INFORMATION MANAGEMENT. II. Specifications and Uses of the Transparent Query Language (TQL)” [1–6] is a continuation of [2] and should be studied immediately after reading PART IIB [2]. It describes (i) the security system that can be easily invoked to deny unauthorized access to any item of information in any database; (ii) the special codes that can be used to specify virtually any degree of uncertainty; (iii) the registry numbers which terminate information paths; and (iv) the command structure for the Transportable Query Language. PART IIC references PART IIA [1], PART IIB [2], PART IID [4] and PART IIF [6]. Concise definitions of Transparent Query Language terms, Conclusions and Acknowledgments are given in PART IIF [6].
    Materialart: Digitale Medien
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  • 8
    ISSN: 1573-8787
    Schlagwort(e): Transparent Query Language ; Mathematically Complete Language ; Philosophically Closed Language ; SOLID Retrieval/Processing System
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Informatik
    Notizen: Abstract In the six parts of the document “HIGH-SPEED TOOLS FOR GLOBAL INFORMATION MANAGEMENT. II. Specifications and Uses of the Transparent Query Language (TQL)” [1–6], the Transparent Query Language (TQL) that is the mathematical basis for the SOLID Retrieval/Processing System [7] is described and its use demonstrated. TQL is directly responsible for the speed, versatility, security and information/question-type independence of the SOLID System. It can be viewed as a Mathematically Complete (or Philosophically Closed) [8] data structure or content/context independent language capable of describing individual or classes of descriptors in any combination with any degree of specificity. The security system is easily used to prevent unauthorized access to any item in any file. TQL is sufficiently general to be used outside the context of information retrieval. It is capable of concisely representing and manipulating a wide variety of time dependent or static numeric and non-numeric information. The six parts of this document [1–6], are as follows. The first part, PART IIA [1], contains a review of the literature and then introduces the Transparent Query Language. It references PART IIB [2], PART IIC [3], PART IID [4], PART IIE [5] and PART IIF [6]. Concise definitions of Transparent Query Language terms, Conclusions and Acknowledgments are given in PART IIF [6]. Section III in PART IIA [1] contains information for converting citations of sections and subsections in the original document to their locations in the partitioned document.
    Materialart: Digitale Medien
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  • 9
    ISSN: 1573-8787
    Schlagwort(e): Transparent Query Language ; Mathematically Complete Language ; Philosophically Closed Language ; SOLID Retrieval/Processing System
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Informatik
    Notizen: Abstract This part, PART IID [4], of the document “HIGH-SPEED TOOLS FOR GLOBAL INFORMATION MANAGEMENT. II. Specifications and Uses of the Transparent Query Language (TQL)” [1–6] is about normalization and manipulation of information representations. It references PART IIA [1], PART IIB [2] and PART IIC [3]. Concise definitions of Transparent Query Language terms, Conclusions and Acknowledgments are given in PART IIF [6].
    Materialart: Digitale Medien
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  • 10
    ISSN: 1573-8787
    Schlagwort(e): Transparent Query Language ; Mathematically Complete Language ; Philosophically Closed Language ; SOLID Retrieval/Processing System ; Sequel (SQL) ; Relational Algebra ; QUEL ; Query-By-Example (QBE)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Informatik
    Notizen: Abstract In this part, PART IIE [5], of the document “HIGH-SPEED TOOLS FOR GLOBAL INFORMATION MANAGEMENT. II. Specifications and Uses of the Transparent Query Language (TQL)” [1–6] the conversion of queries coded in SQL, Relational Algebra, QUEL and Query-By-Examples (QBE) to TQL are demonstrated. PART IIE references PART IIA [1], PART IIB [2], PART IID [3] and PART IIF [6]. Concise definitions of Transparent Query Language terms, Conclusions and Acknowledgments are given in PART IIF [6].
    Materialart: Digitale Medien
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