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  • 1
    Electronic Resource
    Electronic Resource
    The requirement of chrysobactin dependent iron transport for virulence incited by Erwinia chrysanthemi on Saintpaulia ionantha (1991)
    Springer
    Plant and soil 130 (1991), S. 263-271 
    Details
    ISSN: 1573-5036
    Keywords: bacteria ; Erwinia chrysanthemi ; iron transport ; phytopathogenicity ; Saintpaulia ionantha ; virulence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract To incite a systemic disease on its specific host, Saintpaulia ionantha, the soft-rot Erwinia chrysanthemi strain 3937 requires a functional high affinity iron transport system. Under iron starvation, strain 3937 produces chrysobactin, a novel catechol-type siderophore. Recent advances in the biochemistry and genetics of iron assimilation in E. chrysanthemi are reported. Analysis of leaf intercellular fluid from healthy and infected plants suggests: (i) leaf vessels in which the bacteria develop during infection would be low in free iron and (ii) chrysobactin could be produced in planta.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00011882
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Expression, isolation and properties of Fur (ferric uptake regulation) protein ofEscherichia coli K 12 (1988)
    Springer
    BioMetals 1 (1988), S. 62-68 
    Details
    ISSN: 1572-8773
    Keywords: Fur repressor ; Iron regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The clonedfur (ferric uptake regulation) gene ofEscherichia coli K12 was ligated to an expression vector which was inducible with nalidixic acid. The Fur protein was isolated in a single step by immobilized metal-ion-affinity chromatography over zinc iminodiacetate agarose. The amino acid composition of the isolated protein agreed with that predicted from the gene sequence and indicated post-transcriptional removal of the N-terminal methionine residue. All four cysteines were shown to be present as thiols. Proteolysis with trypsin and chymotrypsin yielded large fragments identifiable on polyacrylamide gel electrophoresis. Various divalent metal ions were found by a nitrocellulose filter binding assay to effect non-specific interaction of the Fur dimer with DNA with a dissociation constant of 7 × 10−12 M. A much smaller value, 2.5 × 10−17 M, was measured by gel mobility retardation assay for binding of Fur to a DNA fragment containing the operator sequences of the aerobactin promoter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01128019
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    Paper (German National Licenses)
    Fulltext
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