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  • Key words: Bicameral cultures  (1)
  • Seminal vesicle Epithelium Culture Vasoactive intestinal peptide Receptors/binding sites Secretion Golden hamster  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Polarized epithelial cells of the hamster seminal vesicle in a serum-free bicameral culture system: evidence of secretory and endocytic activities (1995)
    Springer
    Cell & tissue research 282 (1995), S. 181-192 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Bicameral cultures ; Polarized epithelial cells ; Secretion ; Endocytosis ; Seminal vesicle ; Hamster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Primary cultures of epithelial cells from the hamster seminal vesicle were established in a chemically defined medium supplemented with hormones and growth factors. Epithelial cell clusters were prepared combining enzymatic dissociation and mechanical disaggregation and then seeded in bicameral systems equipped with collagen-membrane inserts. A growth curve was generated and the cells were characterized morphologically and morphometrically by light and electron microscopy. The immunocytochemical detection of cytokeratins and the measurement of transepithelial electrical resistance were also performed. The secretory activity was studied by fluorography using L-[35S]methionine as a precursor, and endocytosis was approached using horseradish peroxidase as a tracer. Our results show that epithelial cells of the seminal vesicle can be grown as a monolayer of morphological and functionally polarized cells which retain secretory and endocytic activities. These cell cultures might therefore prove useful to investigate further the regulation of secretion and endocytosis in the seminal vesicle and are a promising model to approach, in a broader scope, cell polarity and protein sorting and targeting.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050471
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Vasoactive intestinal peptide stimulates apical secretion in hamster seminal vesicle epithelial cells in culture (1999)
    Springer
    Cell & tissue research 298 (1999), S. 137-143 
    Details
    ISSN: 1432-0878
    Keywords: Seminal vesicle Epithelium Culture Vasoactive intestinal peptide Receptors/binding sites Secretion Golden hamster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. In this study, we investigated the presence of vasoactive intestinal peptide (VIP) receptors in the epithelial cells of the hamster seminal vesicle, by using cell clusters isolated from the gland and cultivated in a serum-free bicameral culture system. An immunocytochemical approach and autoradiographic and biochemical binding experiments with 125I-VIP as radioligand were performed. The effect of this neuropeptide on cultured cell proliferation and protein secretory activity was also analysed. The release of trichloroacetic-acid-precipitable radioactive material by epithelial cells to the apical and basal compartments of the bi-chamber was estimated in absolute and relative terms. The results of this work indicate that: (1) VIP receptors are present in the membranes of clusters of epithelial cells from seminal vesicles and are further maintained in cultured cells; (2) VIP does not exert any mitogenic effect in these cells; (3) VIP affects the directionality of secretion, favouring the absolute and relative amounts of protein released to the apical compartment of the bi-chamber. The expression of VIP receptors in the epithelial cells of the hamster seminal vesicle and the direct secretagogue-like activity of this neuropeptide in these cells might be affected by other factors, namely, androgens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004419900070
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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