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  • 1
    Electronic Resource
    Electronic Resource
    Deoxyribonucleic acid base composition of simonsiellaceae (1977)
    Springer
    Archives of microbiology 113 (1977), S. 205-207 
    Details
    ISSN: 1432-072X
    Keywords: DNA ; GC content ; Simonsiellaceae ; Simonsiella ; Alysiella
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The molar percentages of guanine plus cytosine in the DNA of 51 strains of Simonsiellaceae were determined by buoyant density ultracentrifugation of cell lysates in CsCl. The DNA base ratios ranged from 41–55 mole-% guanine plus cytosine. These values fall within the range known for the Order Cytophagales, the non-fruiting gliding bacteria, and are out-side the range of the Order Myxobacterales, the fruiting myxobacteria. Among the strains of the genus Simonsiella, four distinct groups can be delineated on the basis of source of origin (sheep, dog, cat, human) and GC content. The neotype of Alysiella filiformis has a GC content of 45.4 mole-%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00492026
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Dorsal-ventral differentiation in Simonsiella and other aspects of its morphology and ultrastructure (1977)
    Springer
    Archives of microbiology 113 (1977), S. 197-204 
    Details
    ISSN: 1432-072X
    Keywords: Gliding bacterium ; Simonsiella ; Oral cavity ; Electron microscopy ; Morphology ; Dorsal-ventral differentiation ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The morphology and ultrastructure of the aerobic, Gram-negative multicellular-filamentous bacteria of the genus Simonsiella were investigated by scanning and transmission electron microscopy. The flat, ribbon-shaped, multicellular filaments show dorsal-ventral differentiation with respect to their orientations to solid substrata. The dorsal surface, orientated away from the substrate, is convex and possesses an unstructured capsule. The ventral surface, on which the organisms adhere and glide, is concave and has an extracellular layer with fibrils extending at right angles from the cell wall. The cytoplasm in the ventral region contains a proliferation of intracytoplasmic membranes and few ribosomes in comparison to the cytoplasm in other parts of the cell. Centripetal cell wall formation is asymmetrical and commences preferentially in the ventral region. Quantitative differences in morphology and cytology exist among selected Simonsiella strains. Functional aspects of this dorsalventral differentiation are discussed with respect to the colonization and adherence of Simonsiella to mucosal squamous epithelial cells in its ecological habitat, the oral cavities of warm-blooded vertebrates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00492025
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Isolation, characterization, and numerical taxonomy of Simonsiella strains from the oral cavities of cats, dogs, sheep, and humans (1978)
    Springer
    Archives of microbiology 118 (1978), S. 235-241 
    Details
    ISSN: 1432-072X
    Keywords: Simonsiella ; Gliding prokaryotes ; Oral flora ; Isolation procedure ; Numerical taxonomy ; Phenogram ; Ecospecies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Forty-nine strains of the gliding prokaryote Simonsiella were isolated from the oral cavities of cats (8), dogs (19), sheep (4), and humans (18) in Southern California by a direct isolation procedure using a complex serum-enriched medium. The numerical taxonomic analysis (unweighted pair-group method using arithmetic averages) of 57 differential traits for each strain was based on standard bacteriological diagnostic tests and included the molar guanine-pluscytosine contents of the DNA and the relative percentages of fatty acid contents reported earlier. The resulting phenogram clustered the strains of Simonsiella into groups that correlated with sources of origin. The study included the neotype strain of Simonsiella crassa (ATCC 27504, ICPB 3651, NCTC 10283) of Australian sheep origin. The strains isolated from dogs, sheep, and humans form clusters of organisms that appear to have become adapted to live in and possibly to have evolved with their respective “hosts”. In our judgment, these source-of-origin clusters represent different “ecospecies”.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00429111
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Fatty acid composition of Simonsiella strains (1977)
    Springer
    Archives of microbiology 113 (1977), S. 209-213 
    Details
    ISSN: 1432-072X
    Keywords: Fatty acid composition ; Simonsiella ; Classification ; Discriminant analysis ; Canonical variables
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Gas-liquid chromatography of methyl esters of bound fatty acids extracted from the cells of 48 Simonsiella strains showed that these aerobic, gliding, multicellular-filamentous bacteria have fatty acid profiles of the pattern considered typical of Gramnegative eubacteria. All strains contained predominantly tetradecanoic acid (29.5%), 9-hexadecenoic acid (22.2%), an unidentified acid with an equivalent chain length of approximately 20 carbon atoms (15.8%), and dodecanoic acid (11.4%). Discriminant analysis of the mean relative percentages of 12 fatty acids correctly assigned 94% of the strains to groups based on their source of origin (i.e., the oral cavities of sheep, cat, human or dog); the relative amounts of only 3 of the fatty acids (9-octadecenoic acid, hexadecanoic acid, and tetradecanoic acid) provided most of this discrimination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00492027
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    Paper (German National Licenses)
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