ISSN:
1573-7217
Keywords:
breast cancer
;
fine needle aspirate cell
;
human papillomavirus
;
PCR
;
Southern blot hybridization
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Oncogenic human papillomavirus (HPV) types 16 and 18 commonly associated with cervical cancer are found in many epithelial malignancies at extra-genital sites including breast. The transforming gene products of HPV have also been shown to immortalize breast epithelial cellsin vitro. But the findings of HPV DNA in breast carcinoma are found to be contradictory. In the present study fine needle aspirate cell (FNAC) samples from 26 breast cancer patients and four breast tumour biopsies were analysed for the presence of HPV 16 and 18 DNA sequences by both polymerase chain reaction (PCR) and Southern blot hybridization. Of 26 fine needle aspi rate cell samples and four breast cancer biopsies, not a single sample was found to be positive by either PCR or Southern blot hybridization. The observation of complete absence of HPV DNA sequences in breast cancer refute the possibility of any role for oncogenic genital HPV types 16 and 18 in the pathogenesis of breast cancer.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01806186
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