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  • 1
    Electronic Resource
    Electronic Resource
    ATP synthesis associated with the conversion of hexachlorocyclohexane related compounds (1982)
    Springer
    Archives of microbiology 131 (1982), S. 330-333 
    Details
    ISSN: 1432-072X
    Keywords: Chlostridium rectum ; Chlorobenzene ; HCH conversion ; Stickland reaction ; ATP synthesis ; Dechlorination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Clostridium rectum strain S-17 converts γ-1,2,3,4,5,6-hexachlorocyclohexane (HCH) related compounds to chlorobenzenes. The metabolites from γ-1,2,3,4,5,6-hexachlorocyclohexene and γ-1,3,4,5,6-pentachlorocyclohexene are identified as 1,2,4-trichlorobenzene and 1,4-dichlorobenzene, respectively. ATP synthesis, converting these chlorinated compounds, is observed in the cell suspension of C. rectum as indicated by luciferase-luciferin reaction and phosphorylation of 32P-labeled phosphate. These observation lead to the conclusion that HCH and related compounds serve as artificial electron acceptors of the Stickland reaction, and therefore, the reductive dechlorination is associated with ATP synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00411180
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Lindane degradation by cell-free extracts of Clostridium rectum (1980)
    Springer
    Archives of microbiology 125 (1980), S. 221-225 
    Details
    ISSN: 1432-072X
    Keywords: Clostridium rectum ; Lindane degradation ; Stickland reaction ; Monochlorobenzene ; Tetrachlorocyclohexene ; Reductive dechlorination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract For lindane degradation, a cell suspension of Clostridium rectum strain S-17 demands the addition of substrates such as leucine, alanine, pyruvate, a leucine-proline mixture, and molecular hydrogen. In the presence of leucine-proline mixture, lindane decomposed in parallel with isovaleric acid formation, and both lindane degradation and isovaleric acid formation were inhibited by monoiodoacetic acid, suggesting a close relation between lindane degradation and the Stickland reaction. Lindane was degraded by cell-free extracts of C. rectum in the presence of dithiothreitol (DTT). Radiogaschromatograms of n-hexane soluble metabolites from [14C] lindane showed the presence of monochlorobenzene and γ-3,4,5,6-tetrachlorocyclohexene, Leucine, NADH, and NADPH were somewhat less active than DTT for lindane degradation in cell-free extracts. Reductive dechlorination seemed the major route of lindane degradation in cell-free extracts as well as in the intact cells of C. rectum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00446880
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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