ISSN:
1437-160X
Keywords:
Anti-lymphocyte antibodies
;
Systemic lupus erythematosus
;
Suppressor T cell
;
Monoclonal antibodies
;
Anti-Ig coated dish
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Anti-lymphocyte antibodies obtained from patients with active systemic lupus erythematosus (SLE) were used in a solid-phase procedure performed at 37 °C to isolate two distinctive T lymphocyte subpopulations. Lymphocytes isolated in this way were characterized by a fivefold enrichment in cells with the T8 phenotype and a threefold diminution in cells with the T4 phenotype over the residual lymphocytes that did not react with the antilymphocyte antibodies. Evidence was obtained that the dominant class of anti-lymphocyte antibodies reacting at 37 °C were of the IgG class. The isolated lymphocyte population, both with and without Con A stimulation strongly suppressed B cell IgG secretion stimulated by pokeweed mitogen. In contrast, the residual cells enhanced Ig secretion in the same system. These results indicate that the positively selected T cells with anti-lymphocyte antibodies from active SLE sera are mainly suppressor T cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00541170
Permalink
