Library

Keywords: Abbreviations ALL Acute lymphoblastic leukemia ; BMT Bone marrow transplantation ; CTL Cytotoxic T-lymphocytes ; IL Interleukin ; MHC Major histocompatibility ; MRD Minimal residual diseaseIntroductionObjective ; The purpose of this protocol is to induce a specific cellular immune reaction after autologous bone marrow transplantation (BMT) by vaccination with human leukocyte antigen (HLA) B13 transfected autologous leukemic cells in children with high-risk relapse of acute lymphoblastic leukemia (ALL). Specific cytotoxic T-lymphocytes (CTL) will be expanded in vivo by low-dose interleukin-2 (IL-2). The expression vector used is the pcDNA3 HLA-B13 plasmid ; driven by a cytomegalovirus promoter. Leukemic cells are transfected by electroporation/ballistomagnetic transfer.