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  • Trichoderma viride  (2)
  • Protoplasts regeneration  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Chemical and structural differences in mycelial and regeneration walls of Trichoderma viride (1975)
    Springer
    Archives of microbiology 105 (1975), S. 277-282 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Trichoderma viride ; Mycelial walls ; Regeneration walls ; Aberrant tubes ; β-(1–3)Glucan ; β-(1–6)Glucan ; Chitin ; Fungal protoplasts ; Protoplasts regeneration
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract When incubated in Winge medium, protoplasts from Trichoderma viride obtained by treatment with Micromonospora chalcea or Streptomyces venezuelae RA lytic systems first synthesized an aberrant wall, different from the normal one; it was aseptate, larger and irregular in size and length. They then regenerated a new wall, similar to the original one from which they were liberated. Analysis showed that the wall polymers were mainly β-(1–3) glucan, β-(1–6) glucan and chitin in the normal walls, whereas chitin was absent in aberrant tubes. These results are discussed below, together with electron micrographs of aberrant and normal walls.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00447147
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Effects of polyoxin D on germination, morphological development and biosynthesis of the cell wall of Trichoderma viride (1976)
    Springer
    Archives of microbiology 108 (1976), S. 183-188 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Trichoderma viride ; Polyoxin D ; Mycelial walls ; Aberrant tubes ; Cell wall synthesis ; Spore germination ; Morphological development ; Regenerating protoplasts ; Chitin ; β-(1-3) Glucan
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract When polyoxin D is added to a spore suspension of Trichoderma viride at a concentration from 50–100 μg/ml, it inhibits from 40–60% of germination. This percentage increases if dimethylsulfoxide (DMSO) is added. Mycelium growing in the presence of polyoxin D becomes irregular and loses its rigidity, showing several bulges along the hypha. Under the electron microscope the features of the cell wall and cytoplasmic content are apparently normal. Nevertheless, after incubation with different lytic systems or with (14C)glucose, it can be seen that polyoxin D partially inhibits the biosynthesis of β-(1-3)glucan and the biosynthesis of chitin to a greater extent attaining inhibition of 83% at 100 μg/ml of the antibiotic concentration. Regenerating protoplasts are less affected by polyoxin D. They do regenerate slower but the percentage of regeneration is more than 80%. Aberrant tubes synthesized by these protoplasts are not affected, they manifest their usual morphology and lack of chitin is confirmed in their composition.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00428949
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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    Artikel (Nationallizenzen)
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