ISSN:
1432-1912
Keywords:
Embryotoxic Actions
;
Trypan Blue
;
Dose Response
;
Trypanblau
;
Embryotoxicität
;
Dosis-Wirkungs-Beziehungen
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The embryotoxic effect of trypan blue on pregnant mice of the NMRI-strain was studied in relation to the dose injected. In addition, the acute toxicity of trypan blue in the adult mouse was determined and compared with the fetal toxicity. The findings were as follows: 1. The acute toxicity of trypan blue given in 1% solution, was determined. The LD50 adult=267 mg/kg when the injection is made subcutaneously. 2. In all treated animals, whatever the size of the injected dose-25 mg/kg, 50 mg/kg, 100 mg/kg or 200 mg/kg-a significant increase is observed both in the overall embryotoxic activity and in the resorption-, malformation- and retardationrates, compared to non-treated animals. Malformation- and retardation-rates as well as the overall embryotoxic activity are found to increase in parallel with increasing dose without a maximum having been attained at 200 mg/kg. In contrast, the rate of resorptions appears to be independent of the dose. 3. The dose that allows 50% of all implantations to evolve into fetuses, showing no recognizable damage-is in the region of 67 mg/kg. This amounts to approximately 1/4 of the LD50 adult
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00997112
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