ISSN:
1432-2307
Schlagwort(e):
Gastric B cell lymphoma
;
T cell receptor repertoire
;
Tumour-infiltrating lymphocyte
;
T cell receptor rearrangement
;
Vβ usage
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Medizin
Notizen:
Abstract Ten cases of primary gastric malignant lymphoma (PGL) were investigated by immunohistochemical and molecular genetic analysis. These cases were diagnosed histopathologically as follicular small cleaved cell type (1 case), diffuse small cleaved cell type (3 cases) and diffuse large cell type (6 cases) based on the WF (Working Formulation) classification. Seven cases classified as small cleaved or diffuse large cell type belong to low (4 cases) or high (3 cases) grade MALT lymphoma according to Isaacson's classification. All PGL belonged to B lineage cells according to immunohistochemical study and immunoglobulin rearrangements. Rearrangements of TCR β chain genes were observed in four of the ten cases. The possibility that the TCR β rearrangements were caused by tumour-infiltrating T-cells (TILs) was supported by the following observations: the tumours did not show T- and B-cell biphenotype, TCR β exhibited functional VDJ rearrangement and Vβ usage pattern was not a neoplastic type. Analysis of the repertoire of the TCR β chain in TILs revealed a common usage of Vβ2 in the above four cases, and furthermore, predominant usage of a particular β chain composed of Vβ2-Dβ2.1-Jβ2.3 was observed in one of the four cases. These results indicate that the TILs of PGL have a restricted TCR repertoire.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00194693
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