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  • 1
    Electronic Resource
    Electronic Resource
    Persistence and recovery of introduced Rhizobium ten years after inoculation on Leucaena leucocephala grown on an Alfisol in southwestern Nigeria (1994)
    Springer
    Plant and soil 159 (1994), S. 199-204 
    Details
    ISSN: 1573-5036
    Keywords: inoculation ; Leucaena leucocephala ; nitrogen fixation ; nodulation ; persistence ; Rhizobium strains
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Establishment of Leucaena leucocephala was poor at Ibadan (Transition forest-savanna zone) and Fashola (savanna zone, 70 km north of Ibadan) in southwestern Nigeria as a result of low soil fertility and the presence of only a few native rhizobia capable of nodulating it. Inoculation with L. leucocephala at these two locations in 1982 resulted in striking responses with Rhizobium strains IRc 1045 and IRc 1050 isolated from L. leucocephala grown in Nigeria. The persistence of inoculated effective Rhizobium strains after inoculation is desirable since it removes the need for reinoculation. Because of the perennial nature of L. leucocephala and its use in long-term alley farming experiments, we examined the persistence of inoculated rhizobial strains after inoculation, and their ability to sustain N2-fixation and biomass production at Ibadan. In 1992, ten years after Rhizobium introduction, uninoculated, L. leucocephala fixed about 150 kg N ha-1 yr-1 or about 41% of total plant N compared to 180 kg N ha-1 yr-1 or 43% measured in 1982. Serological typing of the nodules using the Enzyme-Linked-Immunosorbent Assay (ELISA) and intrinsic resistance to the streptomycin test revealed that most of the nodules (96%) formed on L. leucocephala in 1992 were by Rhizobium strains IRc 1045 and IRc 1050, which were inoculated in 1982. Nodules were absent on uninoculated L. leucocephala grown on the adjacent field with no history of L. leucocephala cultivation. We conclude that the N2 fixed by Rhizobium strains IRc 1045 and IRc 1050 persisted for many years in the absence of L. leucocephala and sustained effectively fixed N2 which growth and yield of L. leucocephala after several years, thus encouraging a possible low-input alley farming system by smallholder farmers in Nigeria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00009281
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  • 2
    Electronic Resource
    Electronic Resource
    Nitrification and mineralization potentials in a limed Ultisol in the humid tropics (1986)
    Springer
    Plant and soil 92 (1986), S. 153-157 
    Details
    ISSN: 1573-5036
    Keywords: Liming ; Nitrate leaching ; Nitrification ; N-mineralization ; Rain forest ; Soil acidity ; Tropics ; Ultisol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary We studied the effect of liming on the rates of mineralization and nitrification in a coarse-textured kaolinitic Ultisol. Soil samples were taken from field plots which received lime rates from 0 to 4mt/ha three years prior to the study. The pH of the soil samples varied from 4.2 to 6.1. Ammonification of soil organic N and added urea source proceeded readily and was not affected by lime rate. Nitrification occurred in both limed and unlimed soils but the rate of nitrification depended upon the rate of lime application. Soil pH, exchangeable Ca and exchangeable A1 were significantly correlated with the amount of NO3-N accumulated at the end of the 65 days incubation period. Nitrification of NH4-N from ammonium sulfate was absent in soils receiving lower rates of lime which gave pH values ranging from 4.2 to 4.8. Added ammonium source was nitrified readily after a 3-week delay period in the soil (pH 6.1) which received a higher rate of lime (4 mt/ha).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02372276
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  • 3
    Electronic Resource
    Electronic Resource
    Nodulation and growth ofLeucaena leucocephala (Lam.) de Wit as affected by inoculation and N fertilizer (1988)
    Springer
    Plant and soil 112 (1988), S. 129-135 
    Details
    ISSN: 1573-5036
    Keywords: alfisol ; inoculation ; Leucaena leucocephala ; nitrogen fertilizer ; nitrogen fixation ; nodulation ; Rhizobium ; shoot growth ; total nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Leonard jar, pot and field experiments examined the effects of inoculation and the influence of nitrogen fertilizer on nodulation, nitrogen fixation and growth ofLeucaena leucocephala (Lam.) de Wit at IITA, Ibadan, Nigeria. Leucaena responded to both inoculation and/or nitrogen application. Shoot growth and total N and P of inoculated plants were comparable to those of the highest N treatment, and the values were about 55% greater than those of uninoculated ones. Field data indicated that toal N yields of inoculated leucaena were increased by 50% with 40 or 80 kg ha−1 of N fertilizer. However, N fertilizer depressed N fixation by 56% as was expected from nodule mass data. N-fixation was delayed for about 8 weeks in the plots without N. Application of small amounts of N starter (20 ppm) proved to be beneficial to satisfy the plant need during the early stage of leucaena growth. The rhizobial strains IRc 1045 and IRc 1050 were effective, competitive and survived well in the field one year after their establishment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02181762
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  • 4
    Electronic Resource
    Electronic Resource
    Nitrogen fixation of field-inoculatedLeucaena leucocephala (Lam.) de Wit estimated by the15N and the difference methods (1989)
    Springer
    Plant and soil 117 (1989), S. 269-274 
    Details
    ISSN: 1573-5036
    Keywords: difference method ; isotope dilution ; Leucaena leucocephala ; nitrogen fixation ; Rhizobium inoculation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The amount of nitrogen fixed byLeucaena leucocephala (Lam.) de Wit was assessed on an Alfisol at the International Institute of Tropical Agriculture located in southwestern Nigeria. Estimated by the difference method, nitrogen fixation of leucaena inoculated with Rhizobium strain IRc 1045 was 133 kg ha−1 in six months. Inoculation with Rhizobium strain IRc 1050 gave a lower nitrogen fixation of 76 kg ha−1. Fertilization with 40 and 80 kg N ha−1 inhibited nitrogen fixation by 43–76% and 49–71%, respectively. Estimates with the15N dilution method gave nitrogen fixation of 134 kg ha−1 in six months when leucaena was inoculated with Rhizobium strain IRc 1045 and 98 kg ha−1 for leucaena inoculated with Rhizobium strain IRc 1050. This nitrogen fixation represented 34–39% of the plant nitrogen. Inoculated leucaena derived 5–6% of its nitrogen from applied fertilizer and 56–54% from soil.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02220721
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  • 5
    Electronic Resource
    Electronic Resource
    Screening woody and shrub legumes for growth, nodulation and nitrogen-fixation potential in two contrasting soils (1996)
    Springer
    Agroforestry systems 33 (1996), S. 137-152 
    Details
    ISSN: 1572-9680
    Keywords: woody legumes ; screening ; Alfisol ; Ultisol ; N2-fixation ; 15N
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In order to identify for alley cropping new candidate species with high biomass and nitrogen-fixing potential, a screening study was conducted on ten woody and shrub legumes (Acacia auriculiformis, Albizia lebbeck, Gliricidia sepium, Leucaena diversifolia, L. leucocephala cv. K28 and cv. K636, Lonchocarpus sericeus, Cajanus cajan, Crotalaria juncea and Tephorsia candida) for 6 months using an acid Ultisol and a non-acid Alfisol. A wide interspecific variability of legumes appeared within soil types, and there were significant species-by-soil interactions for many parameters in this study. In the acid Ultisol, plant growth in height and grith, nodule numbers, nitrogen yield and N2-fixing potential were significantly (P = 0.05) lower than those in the Alfisol. While Albizia lebbeck was outstanding in both acid and non-acid soil conditions for most performance criteria, L. leucocephala cv. K28 was most sensitive to soil acidity with 41.7% of total nitrogen yield in the Ultisol relative to that accumulated in the Alfisol. In addition to L. leucocephala cv. K28 and G. sepium, the most common hedgerow species, A. lebbeck, L. leucocephala cv. K636, L. diversifolia on Alfisol, and A. lebbeck, L. leucocephala cv. K636, L. diversifolia, Tephrosia candida and Cajanus cajan on acid Ultisol, could be considered promising and thus, worthy of further site adaptability trials.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213646
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