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  • 1
    Electronic Resource
    Electronic Resource
    Immunogold localization of nodule-specific uricase in developing soybean root nodules (1986)
    Springer
    Planta 167 (1986), S. 425-436 
    Details
    ISSN: 1432-2048
    Keywords: Bradyrhizobium ; Glycine (uricase in nodules) ; Immunogold labeling ; Nodulin ; Peroxisome biogenesis ; Root nodules ; Uricase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Immunogold labeling was used to study the time of appearance and distribution of a nodule-specific form of uricase (EC 1.7.3.3) in developing nodules of soybean (Glycine max (L.) Merr.) inoculated with Bradyrhizobium japonicum. The enzyme was detected in thin sections of tissue embedded in either L R White acrylic resin or Spurr's epoxy resin, by employing a polyclonal antibody preparation active against a subunit of soybean nodule uricase. Antigenicity was better preserved in L R White resin, but ultrastructure was better maintained in Spurr's. Uricase was first detectable with protein A-gold in young, developing peroxisomes in uninfected cells, coincident with the release of Bradyrhizobium bacteroids from infection threads in adjacent infected cells. As the peroxisomes enlarged, labeling of the dense peroxisomal matrix increased. Gold particles were never observed over the paracrystalline inclusions of peroxisomes, however. Despite a close association between enlarging peroxisomes and tubular endoplasmic reticulum, uricase was not detectable in the latter. In mature nodules, labeling of uricase was limited to the large peroxisomes in uninfected cells. Small peroxisome-like bodies present in infected cells did not become labeled.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391217
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  • 2
    Electronic Resource
    Electronic Resource
    Cellular compartmentation of ureide biogenesis in root nodules of cowpea (Vigna unguiculata (L.) Walp.) (1987)
    Springer
    Planta 172 (1987), S. 162-175 
    Details
    ISSN: 1432-2048
    Keywords: Nitrogen fixation ; Peroxisome ; Root nodules ; Ureide biogenesis ; Uricase ; Vigna
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cowpea (Vigna unguiculata (L.) Walp.) nodules have been investigated by means of cytochemical and immunocytochemical procedures at the ultrastructural level in order to assess the role of the uninfected cells in ureide biogenesis. Uricase activity in the nodules was shown by cytochemical methods to be localized exclusively in the numberous large peroxisomes confined to the uninfected cells; the small peroxisomes in the infected cells did not stain for uricase. Uricase was also localized in the peroxisomes of uninfected cells by immunogold techniques employing polyclonal antibodies against nodule-specific uricase of soybean. There was no labeling above background of any structures in the infected cells. The results indicate that the uninfected cells are essential for ureide biogenesis in cowpea. Although tubular endoplasmic reticulum, the presumptive site of allantoinase, increases greatly in the uninfected cells during nodule development, it virtually disappears as the nodules mature. The inconsistency between the disappearance of the tubular endoplasmic reticulum from older nodules and the high allantoinase activity reported for older plants remains to be explained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00394584
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Specialization for ureide biogenesis in the root nodules of black locust (Robinia pseudoacacia L.), an amide exporter (1990)
    Springer
    Protoplasma 157 (1990), S. 102-111 
    Details
    ISSN: 1615-6102
    Keywords: Peroxisomes ; Robinia ; Root-nodules ; Ureide biogenesis ; Uricase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Black locust (Robinia pseudoacacia L.), a member of the legume tribe Robinieae, has indeterminate root nodules and is primarily an amide exporter. However, in greenhouse-grown seedlings inoculated with rhizobia, ureide-N comprises approximately 8% of the total soluble-N in the xylem sap. Ultrastructurally, young interstitial cells (i.e., uninfected cells in the infected region near the nodule meristem) develop enlarged peroxisomes and abundant tubular ER, properties heretofore found to be characteristic only of members of the Phaseoleae, which have determinate nodules and are exporters principally of ureides. Many of the peroxisomes in the interstitial cells of black locust nodules react strongly for uricase (EC 1.7.3.3) activity in a cytochemical test employing diaminobenzidine, but some of the peroxisomes, particularly those farther back from the meristem, react weakly or not at all, even though enlarged. The ultrastructural specializations of the interstitial cells seem disproportionately large in comparison to the relatively low levels of ureides in the xylem transport stream. We suggest that similar specialization of some of the interstitial cells might be found in other legumes that export low amounts of ureides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01322642
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  • 4
    Electronic Resource
    Electronic Resource
    Cytochemical localization of uricase and catalase in developing root nodules of soybean (1987)
    Springer
    Protoplasma 140 (1987), S. 1-12 
    Details
    ISSN: 1615-6102
    Keywords: Cerium chloride ; Diaminobenzidine (DAB) ; Peroxisomes ; Root-nodules ; Soybean ; Uricase cytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two different cytochemical methods were used to study the localization of uricase (EC 1.7.3.3) and catalase (EC 1.11.1.6) in developing root nodules of soybean (Glycine max) inoculated as seeds withBradyrhizobium japonicum. One of the methods employs DAB (3,3′-diaminobenzidine) and detects uricase activity indirectly by coupling it to endogenous catalase activity. The other method utilizes cerium chloride to detect uricase activity directly. These methods were modified to obtain not only a strong staining reaction but also improved ultrastructural preservation. With the indirect DAB method, intense staining indicative of both uricase and catalase activity was obtained in the enlarged peroxisomes of older uninfected cells. Similar staining was observed in enlarging peroxisomes of younger uninfected cells, and in the material of associated sacs whose bounding membranes appear to arise as distensions of the ER. The observations are discussed in relation to the controversial role of the ER in peroxisome biogenesis. Although the small peroxisome-like organelles of infected cells did not give a clearly positive reaction in the indirect DAB method, they reacted positively in the cerium chloride method, and are considered to be peroxisomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01273250
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    Paper (German National Licenses)
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