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  • Artikel: DFG Deutsche Nationallizenzen  (4)
  • Horseradish shoot primordia  (2)
  • Viscosity  (2)
  • 1
    Digitale Medien
    Digitale Medien
    Cryopreservation of encapsulated shoot primordia induced in horseradish (Armoracia rusticana) hairy root cultures (1997)
    Springer
    Plant cell reports 16 (1997), S. 469-473 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Key words Cryopreservation ; Encapsulation-dehydration ; Encapsulation-vitrification ; Hairy roots ; Horseradish shoot primordia
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Shoot primordia induced in Armoracia rusticana Gaertn. Mey. et Scherb. (horseradish) hairy root cultures were successfully cryopreserved by two cryogenic procedures. Encapsulated shoot primordia were precultured on solidified Murashige-Skoog medium supplemented with 0.5 M sucrose for 1 day and then dehydrated with a highly concentrated vitrification solution (PVS2) for 4 h at 0°C prior to a plunge into liquid nitrogen. The survival rate of encapsulated vitrified primordia amounted to 69%. In a revised encapsulation-dehydration technique, the encapsulated shoot primordia were precultured with a mixture of 0.5 M sucrose and 1 M or 1.5 M glycerol for 1 day to induce dehydration tolerance and then subjected to air-drying prior to a plunge into liquid nitrogen. The survival rate of encapsulated dried primordia was more than 90%, and the revived primordia produced shoots within 2 weeks after plating. A long-term preservation of shoot primordia was also achieved by the technique. Thus, this revised encapsulation-dehydration technique appears promising as a routine method for the cryopreservation of shoot primordia of hairy roots.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01092768
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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    Artikel (Nationallizenzen)
    Volltext
  • 2
    Digitale Medien
    Digitale Medien
    Cryopreservation of encapsulated shoot primordia induced in horseradish (Armoracia rusticana) hairy root cultures (1997)
    Springer
    Plant cell reports 16 (1997), S. 469-473 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Cryopreservation ; Encapsulation-dehydration ; Encapsulation-vitrification ; Hairy roots ; Horseradish shoot primordia
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Shoot primordia induced inArmoracia rusticana Gaertn. Mey. et Scherb. (horseradish) hairy root cultures were successfully cryopreserved by two cryogenic procedures. Encapsulated shoot primordia were precultured on solidified Murashige-Skoog medium supplemented with 0.5M sucrose for 1 day and then dehydrated with a highly concentrated vitrification solution (PVS2) for 4 h at 0°C prior to a plunge into liquid nitrogen. The survival rate of encapsulated vitrified primordia amounted to 69%. In a revised encapsulation-dehydration technique, the encapsulated shoot primordia were precultured with a mixture of 0.5M sucrose and 1M or 1.5M glycerol for 1 day to induce dehydration tolerance and then subjected to air-drying prior to a plunge into liquid nitrogen. The survival rate of encapsulated dried primordia was more than 90%, and the revived primordia produced shoots within 2 weeks after plating. A long-term preservation of shoot primordia was also achieved by the technique. Thus, this revised encapsulation-dehydration technique appears promising as a routine method for the cryopreservation of shoot primordia of hairy roots.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01092768
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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    Artikel (Nationallizenzen)
    Volltext
  • 3
    Digitale Medien
    Digitale Medien
    Viscoelastic properties of middle ear effusions from pediatric otitis media with effusion and their relation to gross appearance (1990)
    Springer
    European archives of oto-rhino-laryngology and head & neck 247 (1990), S. 60-62 
    Details
    ISSN: 1434-4726
    Schlagwort(e): Viscosity ; Elasticity ; Otitis media with effusion ; Mucociliary transport
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The viscosity and the elasticity were measured in 146 individual middle ear effusions (MEEs) from 93 children with otitis media with effusion (OME). The effusions were classified into serous or mucoid type by their macroscopic appearance. The distribution of elasticity showed two peaks, corresponding to the peaks of serous and mucoid types of effusions. There were a small number of effusions in the intermediate range of elasticity, where the effusions were most effectively transported by the cilia. These findings suggests that the MEEs of pediatric OME are accumulations of rheologically suboptimal fluid in the middle ear cavity.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00240953
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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    Artikel (Nationallizenzen)
    Volltext
  • 4
    Digitale Medien
    Digitale Medien
    The effect of an orally administered proteolytic enzyme on the elasticity and viscosity of nasal mucus (1988)
    Springer
    European archives of oto-rhino-laryngology and head & neck 244 (1988), S. 355-359 
    Details
    ISSN: 1434-4726
    Schlagwort(e): Chronic sinusitis ; Proteolytic enzyme ; Mucus ; Viscosity ; Elasticity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary We have evaluated the effect of serratiopeptidase (SER), a proteolytic enzyme, on the elasticity and viscosity of the nasal mucus in adult patients with chronic sinusitis. SER was administered in a dose of 30mg/day orally for 4 weeks. Nasal mucus was collected from the nasal cavities of each patient before (week 0) and 4 weeks after the start of the medication (week 4). The storage modulus (G′) and the dynamic viscosity (η′) of each specimen of nasal mucus were determined by an oscillating sphere magnetic rheometer at frequencies of 0.5, 1, 5, 10 and 20 Hz at a constant temperature of 25° C. The dynamic viscosity (η′) of the mucus at week 4 was significantly lower than that at week 0 (at frequencies of 5, 10 and 20 Hz). No significant differences were observed in the storage modulus (G′) between the mucus at week 0 and week 4. SER reduced the viscosity but not the elasticity of the nasal mucus. These findings are discussed in relation to mucociliary clearance.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00497464
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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    Artikel (Nationallizenzen)
    Volltext
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