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  • 1
    Digitale Medien
    Digitale Medien
    Localization of iron-superoxide dismutase in the cyanobiont ofAzolla filiculoides Lam (1992)
    Springer
    Protoplasma 169 (1992), S. 1-8 
    Details
    ISSN: 1615-6102
    Schlagwort(e): Immunogold labeling ; Iron-superoxide dismutase ; Cyanobiont ; Azolla filiculoides ; Heterocysts ; Nitrogenase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Immunogold labeling and transmission electron microscopy were used to localize iron-superoxide dismutase (Fe-SOD) in the different cells of nitrogen-fixing cyanobacterial symbiont present within different leaf cavity groups ofAzolla filiculoides Lam. As evidenced by Western blotting and immunoprecipitation, Fe-SOD antibody fromAnabaena cylindrica recognized Fe-SOD in extracts of the cyanobiont and showed the same electrophoretic mobility and pattern as purifiedA. cylindrica Fe-SOD. In vegetative cells of the cyanobiont, Fe-SOD was mainly localized in the thylakoidal membranes and in the outer membrane. The labeling pattern was similar in vegetative cells of the various groups of leaf cavities examined except at the apex where a lower gold particle density was seen. In heterocysts of the leaf cavity groups containing high nitrogenase activity, Fe-SOD labeling was most pronounced and more intense than in vegetative cells. The Fe-SOD label was preferentially located throughout the heterocyst cytoplasm and in the honeycomb regions. In accordance with the decline in nitrogenase activity, the Fe-SOD gold particle density decreased significantly in heterocysts of basal leaf cavity group. The presence of Fe-SOD in regions of high nitrogenase protein levels, and the fact that the pattern of Fe-SOD label parallels that of nitrogenase activity support a role of Fe-SOD in the protection of nitrogenase against superoxide radicals.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01343364
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    In vitro development of parthenocarpic fruits of Crocus sativus L. (1987)
    Springer
    Plant cell, tissue and organ culture 11 (1987), S. 75-78 
    Details
    ISSN: 1573-5044
    Schlagwort(e): Crocus sativus L. fruit development in vitro ; parthenocarpy ; 2,4-dichlorophenoxyacetic acid ; benzyladenine ; gibberellin ; abscisic acid
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract In vitro development of parthenocarpic fruits of Crocus sativus L. was induced by culturing ovaries on MS agar medium supplemented with growth-regulators (2,4-D, GA3 and BAP). Amongh these, 2,4-D was the most effective in promoting fructification. The fructigenic activity was independent of both the stage at which the ovaries were excised (before, during or after anthesis) and pollination of the stigmas. Unlike the above compounds, abscisic acid inhibited fructification.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00036578
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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