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  • 1
    Digitale Medien
    Digitale Medien
    The renal subcapsular site offers better growth conditions for transplanted mouse pancreatic islet cells than the liver or spleen (1986)
    Springer
    Diabetologia 29 (1986), S. 670-672 
    Details
    ISSN: 1432-0428
    Schlagwort(e): Mouse panreatic islets ; islet transplantation ; implantation sites ; autoradiography ; labelling index ; islet cell replication
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary In order to investigate the importance of the transplantation site for the replication of grafted islet cells, we implanted syngeneic mouse pancreatic islets intrasplenically, intraportally and subcapsularly in the kidney. Fourteen days later the alloxan-diabetic mice were killed after an injection of tritiated thymidine, and the graft-bearing organs fixed and autoradiography. The highest labelling indices were recorded for subcapsularly grafted islets, followed by intraportal and intrasplenic islets in that order. In separate experiments some islet-containing kidney sections were immune stained for insulin before the autoradiographic process. The labelling index of the insulin-positive cells was as high as in the entire islet cell population of the sections from the same mice stained with haematoxylin only. This indicates that the B cells of the islets replicate as often as the other islet cell types. The present data also suggest that the renal subcapsular space offers better growth conditions for transplanted islet cells than the liver or spleen.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00869269
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Rapid deposition of amyloid in human islets transplanted into nude mice (1995)
    Springer
    Diabetologia 38 (1995), S. 543-549 
    Details
    ISSN: 1432-0428
    Schlagwort(e): Islet amyloid polypeptide ; human pancreatic islets ; islet transplantation ; immune histochemistry ; hyperglycaemia
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Human islets of Langerhans were transplanted to the subcapsular space of the kidneys of nude mice which were either normoglycaemic or made diabetic with alloxan. After 2 weeks, the transplants were processed for light and electron microscopical analyses. In all transplants, islet amyloid polypeptide (IAPP)-positive cells were found with highest frequency in normoglycaemic animals. IAPP-positive amyloid was seen in 16 out of 22 transplants (73%), either by polarisation microscopy after Congo red staining or by immune electron microscopy. At variance with previous findings of amyloid deposits exclusively in the extracellular space of islets of non-insulin-dependent diabetic patients, the grafted islets contained intracellular amyloid deposits as well. There was no clear difference in occurrence of amyloid between diabetic and non-diabetic animals. The present study indicates that human islets transplanted into nude mice very soon present IAPP-positive amyloid deposits. This technique may provide a valuable model for studies of the pathogenesis of islet amyloid and its impact on islet cell function.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00400722
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  • 3
    Digitale Medien
    Digitale Medien
    Failure of successful intrasplenic transplantation of islets from lean mice to cure obese-hyperglycaemic mice, despite islet growth (1981)
    Springer
    Diabetologia 20 (1981), S. 237-241 
    Details
    ISSN: 1432-0428
    Schlagwort(e): Obese-hyperglycaemic mice ; isolated pancreatic islets ; islet transplantation ; serum glucose ; islet volume ; autoradiography ; islet cell replication ; immunocytochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Implantation of allogeneic pancreatic islets encapsulated in Millipore diffusion chambers has been reported to normalize the obese-hyperglycaemic syndrome in mice. In the present study, both young and adult ob/ob mice remained hyperglycaemic and gained weight after intrasplenic implantation of 500 isogeneic islets isolated from lean mice. Such islets normalized the elevated blood-glucose of alloxan-diabetic lean mice. Morphometric analysis of the intrasplenically implanted islets showed that the mean islet volume in the ob/ob mice was five times larger than that of the lean, non-diabetic mice. Immunocytochemical staining of the spleens showed an increased proportion of B-cells in the enlarged, intrasplenic islets in the ob/ob mice. Moreover, autoradiographical examination of these islets demonstrated the presence of several labelled cells. These results suggest that the growth of the implanted “lean” islets is due to extrapancreatic factors which stimulate islet cell replication in the obese-hyperglycaemic mouse.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00252634
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  • 4
    Digitale Medien
    Digitale Medien
    Rapid deposition of amyloid in human islets transplanted into nude mice (1995)
    Springer
    Diabetologia 38 (1995), S. 543-549 
    Details
    ISSN: 1432-0428
    Schlagwort(e): Key words Islet amyloid polypeptide ; human pancreatic islets ; islet transplantation ; immune histochemistry ; hyperglycaemia.
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Human islets of Langerhans were transplanted to the subcapsular space of the kidneys of nude mice which were either normoglycaemic or made diabetic with alloxan. After 2 weeks, the transplants were processed for light and electron microscopical analyses. In all transplants, islet amyloid polypeptide (IAPP)-positive cells were found with highest frequency in normoglycaemic animals. IAPP-positive amyloid was seen in 16 out of 22 transplants (73 %), either by polarisation microscopy after Congo red staining or by immune electron microscopy. At variance with previous findings of amyloid deposits exclusively in the extracellular space of islets of non-insulin-dependent diabetic patients, the grafted islets contained intracellular amyloid deposits as well. There was no clear difference in occurrence of amyloid between diabetic and non-diabetic animals. The present study indicates that human islets transplanted into nude mice very soon present IAPP-positive amyloid deposits. This technique may provide a valuable model for studies of the pathogenesis of islet amyloid and its impact on islet cell function. [Diabetologia (1995) 38: 543–549]
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00400722
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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