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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 135 (1983), S. 110-114 
    ISSN: 1432-072X
    Keywords: Emulsifying agent ; 2,4,5-Trichlorophenoxy-acetic acid ; Pseudomonas cepacia AC1100
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The culture supernatant of Pseudomonas cepacia (ATCC 39027) grown on 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) was found to contain an agent which can emulsify 2,4,5-T. The emulsion is stable for several hours. The emulsifying agent was produced in response to growth on 2,4,5-T, although some emulsification was observed in culture supernatant of glucose grown cells. The emulsifying agent is most active with 2,4,5-T but has some activity towards other chlorinated compounds such as chlorophenols. In growing culture the emulsifying agent adheres to the cell surface as a slimy layer. The emulsifying agent is believed to have a role in transport of 2,4,5-T into the cells.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of industrial microbiology and biotechnology 15 (1995), S. 162-168 
    ISSN: 1476-5535
    Keywords: biofilm anchoring ; alginate gene activation ; biofilm detachment ; alginate lyase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Pseudomonas aeruginosa synthesizes an exopolysaccharide called alginate in response to environmental conditions. Alginate serves to protect the bacteria from adversity in its surroundings and also enhances adhesion to solid surfaces. Transcription of the alginate biosynthetic genes is induced upon attachment to the substratum and this leads to increased alginate production. As a result, biofilms develop which are advantageous to the survival and growth of the bacteria. In certain circimstances,P. aeruginosa produces an alginate lyase enzyme which cleaves the polymer into short oligosaccharides. This negates the anchoring properties of the alginate and results in increased detachment of the bacteria away from the surface, allowing them to spread and colonize new sites. Thus, both alginate biosynthetic and degradative enzymes are important for the development, maintenance and spread ofP. aeruginosa biofilms.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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