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  • In situ hybridization  (2)
  • evolutionary algorithms  (2)
  • 1
    ISSN: 1432-2242
    Keywords: rDNA sites ; Centromeric repetitive DNA ; Telomere ; In situ hybridization ; Southern hybridization ; Ag-NOR ; Cowpea ; Physical maps
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A knowledge of genome organization is important for understanding how genomes function and evolve, and provide information likely to be useful in plant breeding programmes involving hybridization and genetic manipulation. Molecular techniques, including in situ hybridization, molecular cloning and DNA sequencing, are proving valuable tools to investigate the structure, organization, and diversity of chromosomes in agricultural crops. Heterologous labelled 18 s-5.8 s-25 s (pTa71) and 5 s rDNAs (pTa794) were used for in situ hybridization on Vigna unguiculata (L.) Walp. chromosomes. Hybridization with 18 s-5.8 s-25 s rRNA gene probes occurred at the same chromosomal sites which were positive to the CMA fluorochrome. Silver staining of nucleolar-organizing regions indicated that all the rDNA sites detected using the 18 s-5.8 s-25 s rRNA gene probe possessed active genes. Degenerate telomeric repeats gave hybridization signals at the telomeres of most chromosomes and no intercalary sites were detected at metaphase; the sequences appear to have no preferential distribution in interphase nuclei. A repetitive DraI family from V. unguiculata was cloned (pVuKB1) and characterized. The DraI repeat is 488 nucleotides long, AT rich (74%), and hybridized on all chromosomes in the centromeric areas. The presence of this sequence family was investigated by Southern hybridization in different Vigna species and other Leguminoseae. It was only detected in V. unguiculata, and hence represents a species-specific DNA sequence.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of intelligent manufacturing 9 (1998), S. 331-338 
    ISSN: 1572-8145
    Keywords: Simulation ; modelling ; machine learning ; evolutionary algorithms ; artificial neural network
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The use of simulation technology as a tool for planning and control is of increasing significance in most fields of production. The main part of the expenditure concerning simulation analyses is the modelling of the considered production. Despite the use of modern building-block-oriented modelling technology, this modelling can often not be done by the user, but only by external experts. Against this backdrop, an adaptive simulation system is being developed by the Institute for Industrial Manufacturing and Management (IFF) at the University of Stuttgart. It independently adapts to real production processes, i.e. it learns about the interdependencies of production processes, and, in this way, supports the user in constructing and maintaining the model. In terms of information technology, the research in the field of artificial intelligence, especially in the subdomain of machine learning, is the basis for the realization of such adaptive systems.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Journal of intelligent manufacturing 9 (1998), S. 289-294 
    ISSN: 1572-8145
    Keywords: Manufacturing process chain ; modelling ; optimization ; neural networks ; evolutionary algorithms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Today's manufacturing methods are caught between the growing need for quality, high process safety, minimal manufacturing costs, and short manufacturing times. In order to meet these demands, process setting parameters have to be chosen in the best possible way, according to demand on quality. For such optimization it is necessary to represent the processes in a model. Due to the enormous complexity of many processes and the high number of influencing parameters, however, conventional approaches to modelling and optimization are no longer sufficient. In this article it is shown how, by means of applying neural networks for process modelling, even these highly complex interdependencies can be learned. That way both process and quality parameters can be assessed before or during processing. By connecting them with corresponding cost models, it is possible to optimize processes with the help of evolutionary algorithms. Using examples of different manufacturing processes, the possi bilities for process modelling and optimization with neural networks and evolutionary algorithms are demonstrated.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 244 (1994), S. 420-425 
    ISSN: 1617-4623
    Keywords: Pennisetum glaucum ; Satellite DNA ; In situ hybridization ; Centromeric heterochromatin ; Methylation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A HaeIIl monomer of a repetitive DNA family from Pennisetum glaucum (L.) R. Br. cv. Massue has been cloned and characterized. The repeat is 137 bp long and is organized in head-to-tail orientation in tandem arrays. The HaeIII monomer contains 55% A+T residues. The distribution of this highly repetitive sequence in different Pennisetum species and in other cereals was investigated. The HaeIII satellite is present in all Pennisetum species investigated but absent from other genera examined. In situ hybridization revealed a centromeric localization of this sequence on all seven chromosome pairs and indicated chromosome-specific differences in copy number. Methylation was investigated by comparative restriction enzyme analysis (Msp/HpaII) which showed a greater extent of methylation of the internal C of the enzyme recognition site 5′-CCGG. A South-Western analysis, using an anti-methylcytosine antibody to examine the methylation status in P. glaucum confirmed that the sequence is not highly methylated.
    Type of Medium: Electronic Resource
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