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  • 1
    Electronic Resource
    Electronic Resource
    Intestinal mucosal lipid peroxidation and absorptive function In Salmonella typhimurium mediated intestinal infection (1998)
    Springer
    Molecular and cellular biochemistry 178 (1998), S. 345-352 
    Details
    ISSN: 1573-4919
    Keywords: Salmonella typhimurium ; brush border membrane ; lipid peroxidation ; free radicals ; antioxidants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract S. typhimurium infection is associated with neutrophil infiltration within the intestinal mucosa. Neutrophil activation provides a major source of reactive oxygen species (ROS). The mucosal pathology of S. typhimurium infection may be in part due to the excessive production of these reactive species. This study was carried out to investigate if ROS play a role in mediating the changes in the structural components and functional properties of brush border membrane (BBM) in rats during S. typhimurium infection. This was done by determining the changes in the BBM extent of lipid peroxidation and absorptive function. A significant increase in the extent of lipid peroxidation of BBM during S. typhimurium infection was observed as judged by malondialdehyde (MDA) and conjugated diene formation and depletion of α-tocopherol and protein associated thiol groups. A significant decrease in the BBMV (brush border membrane vesicle) transport of amino acids was also observed. However there was no change in the transport of D-glucose. The decrease in amino acid transport further led to a significant decrease in the enterocyte level of protein synthesis. Exposure of BBMV to a free radical donor, cumene hydroperoxide, also led to an increase in the extent of lipid peroxidation and a decrease in the amino acid transport. Possibly ROS might play a significant role in mediating the mucosal damage during S. typhimurium infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006891019115
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  • 2
    Electronic Resource
    Electronic Resource
    Raman spectral studies of ion-molecular interactions of lithium bromide and lithium iodide with dimethyl sulphoxide (1989)
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 20 (1989), S. 169-179 
    Details
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Raman spectra in the C—H and S=O stretching and S=O bending regions are reported for solutions of lithium halides and tetrabutylammonium bromide (TBAB) in dimethyl sulphoxide (DMSO) and DMSO-d6. The C—D stretching band of DMSO-d6 shows two components in solutions of TBAB with DMSO-d6, whereas the C—H stretching band of DMSO for solutions of LiX (X = Br and I) in DMSO show some broadening and asymmetry in the low-frequency region. The S=O stretching bands show changes in band shapes and band contours in solutions of electrolytes in DMSO. Fourier deconvolution of C—H and S=O stretching bands were carried out to gain more information regarding number of components present under the broad structured bands. All the solutions show a significant decrease in the intensity of the S=O bending bands when compared with the neighbouring C—S bending band. The results are discussed in terms of anion- and cation-molecular interactions with DMSO.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jrs.1250200308
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  • 3
    Electronic Resource
    Electronic Resource
    Raman spectral studies of aqueous solutions of non-electrolytes: Dimethylsulfoxide, acetone and acetonitrilePresented at 6th International Conference on Raman Spectroscopy, Bangalore, India (1978). (1982)
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 13 (1982), S. 178-188 
    Details
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Raman spectra of aqueous solutions of dimethylsulfoxide, dimethylsulfoxide-d6, acetone and acetonitrile are reported in the OH stretching region, and in the stretching regions of the functional groups of these solutes. Raman intensities and frequency shifts of these bands show definite trends with varying concentrations of the solutions. The results obtained are discussed in terms of the mixture model of water, and they are compared with results obtained from Raman spectral studies of aqueous electrolytes. It is proposed that water structure breaking and the formation of solute-solvent complexes when organic solutes are added to water can account for these results.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jrs.1250130214
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  • 4
    Electronic Resource
    Electronic Resource
    Self-association of dimethyl sulphoxide and its dipolar interactions with water: Raman spectral studies (1984)
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 15 (1984), S. 80-85 
    Details
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Raman spectra of solutions of dimethyl sulphoxide (DMSO) in carbon tetrachloride (CCl4) and water are reported for both parallel and perpendicular polarizations in the S=O stretching region. The various bands obtained were subjected to Gaussian band fittings. It was observed that the bands for solutions in CCl4 may be fitted satisfactorily by considering four components and those for solutions in water may be fitted by considering five components. It is inferred that the band at ca 1070 cm-1 observed in the spectra of DMSO in CCl4 belongs to the S=O stretching mode of free DMSO, whereas the two bands observed at ca 1058 cm-1 and ca 1040 cm-1 belong to out-of-phase and in-phase S=O stretchings of the cyclic dimer. The low-frequency band at ca 1025 cm-1 is assigned to linear dimer/higher polymers. Aqueous solutions of DMSO show an additional band at ca 1010 cm-1 due to DMSO hydrogen-bonded with water.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jrs.1250150203
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