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  • 1990-1994  (3)
  • fucose  (2)
  • 07.65.-b  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Temperature measurement using degenerate four-wave mixing with non-saturating laser powers (1994)
    Springer
    Applied physics 58 (1994), S. 459-466 
    Details
    ISSN: 1432-0649
    Keywords: 82.40.Py ; 07.20.Dt ; 07.65.-b ; 33.20.Lg
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We report the use of Degenerate Four-Wave Mixing (DFWM) in the OHA 2 Σ + ←X 2 ∏ (υ′ = 0 ←υ″ = 0) band for temperature determination in a propane/air flame using laser powers below the saturation level. We show that at these low power levels the dependence of the signal on the dipole moment for the transition has to be established before meaningful temperature data can be obtained. This presents a paradox in that the temperature has to be known before the form of the dependence on the dipole moment can be determined. Solutions to this paradox are presented. We also show that absorption of the laser beams in this OH band system cannot be neglected and that failure to correctly account for the absorption leads to a large over estimate of the flame temperature. Furthermore, we show that the accuracy of the absorption-corrected temperature is critically dependent on the accuracy with which the measurement position within the flame is known. Finally, the temperature calculated from DFWM spectra using the correct dipole moment power and absorption is compared to the temperatures obtained using Laser-Induced Fluorescence (LIF) and Coherent Anti-Stokes Raman Spectroscopy (CARS).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01081076
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    R-phycoerythrin from the macroalgaCorallina officinalis (Rhodophyceae) and application of a derived phycofluor probe for detecting sugar-binding sites on cell membranes (1991)
    Springer
    Journal of applied phycology 3 (1991), S. 345-354 
    Details
    ISSN: 1573-5176
    Keywords: Corallina officinalis ; phycoerythrin ; fucose ; flow cytometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract R-Phycoerythrin (absorption spectrum 280; 495;sh 535, 564 nm with A564/A280 ratio of 5.3) was purified from the red macroalgaCorallina officinalis. The relative molecular mass determined from PAGE was 240 000. SDS-PAGE demonstrated two major subunits ofM r 20 000 and 21 000, respectively, and a minor subunit ofM r 30 000. A fucospyranosyl phenylisothiocyanate conjugate was prepared and this novel fluorescent affinity reagent used in conjunction with a flow cytometer to probe fucose-binding sites on blood mononuclear cells. By varying the sugar and using other phycobiliproteins the approach has the potential for simultaneously monitoring different sugar binding sites on subsets of cells within populations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00026097
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    R-phycoerythrin from the macroalgaCorallina officinalis (Rhodophyceae) and application of a derived phycofluor probe for detecting sugar-binding sites on cell membranes (1991)
    Springer
    Journal of applied phycology 3 (1991), S. 345-354 
    Details
    ISSN: 1573-5176
    Keywords: Corallina officinalis ; phycoerythrin ; fucose ; flow cytometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract R-Phycoerythrin (absorption spectrum 280; 495;sh 535, 564 nm with A564/A280 ratio of 5.3) was purified from the red macroalgaCorallina officinalis. The relative molecular mass determined from PAGE was 240 000. SDS-PAGE demonstrated two major subunits ofM r 20 000 and 21 000, respectively, and a minor subunit ofM r 30 000. A fucospyranosyl phenylisothiocyanate conjugate was prepared and this novel fluorescent affinity reagent used in conjunction with a flow cytometer to probe fucose-binding sites on blood mononuclear cells. By varying the sugar and using other phycobiliproteins the approach has the potential for simultaneously monitoring different sugar binding sites on subsets of cells within populations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02392888
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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