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  • Artikel: DFG Deutsche Nationallizenzen  (266)
  • Chemistry  (259)
  • genetics  (7)
  • 1
    ISSN: 0959-8103
    Schlagwort(e): Pseudomonas putida ; poly(β-hydroxyalkanoate) ; microbial polyesters ; poly[3-hydroxy-6(4-cyanophenoxy)hexanoate] ; non-linear optics ; in-vivo biodegradation ; Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Maschinenbau , Physik
    Notizen: Pseudomonas putida KT 2442 was utilized as biocatalyst to form optoactive poly(β-hydroxyalkanoate)s (PHAs) from a cosubstrate mixture of octanoate and the achiral polarizable carbon source 6(4-cyanophenoxy)hexanoate, CPH. COSY and heteronuclear multiplet quantum correlation experiments were used to assign 1H and 13C NMR signals of 3-hydroxy-6(4-cyanophenoxy)hexanoate (3HCPH) repeat units. The methine carbon of 3HCPH repeat units was sensitive to repeat unit sequence effects, indicating that a substantial fraction of 3HCPH centered triad sequences in the product contain neighboring 3-hydroxyoctanoate and 3-hydroxhexanoate repeat units. Comparing the thermal properties of 0 and 19.6 mol% 3HCPH samples by differential scanning calorimetry shows that 3HCPH incorporation results in melting at temperatures 〉64°C (not seen for the 0 mol% sample), more rapid crystallization and a new Tg transition at ∼ -21°C. These characteristics indicate that chains and/or chain segments are formed that are enriched in 3HCPH which phaseseparate and form a unique crystal structure. Measurements of second harmonic generation (SHG) intensities carried out using in-situ corona-poled samples showed weak SHG signals that increased by a factor of 8 for an increase in the 3HCPH content from 26 to 34 mol%. Comparatively higher SHG intensities (5 times) were found for PHAs which contained 5.1 mol% 3-hydroxy-6(4-nitrophenoxy)hexanoate (3HNPH) repeat units relative to a PHA with 17 mol% 3HCPH. In-vivo biodegradation studies of microbial polyesters prepared with and without 3HCPH repeat units showed that PHA chains with 3HCPH degraded to lesser extents (weight loss of ∼ 20 and 50% over 72 h incubations). The large increase in polydispersity from 1.9 to 4.3 observed during in-vivo biodegradation of microbial polyesters containing 3HCPH repeat units was attributed to the existence of chains with highly variable contents of 3HCPH repeat units.
    Zusätzliches Material: 9 Ill.
    Materialart: Digitale Medien
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Behavior genetics 12 (1982), S. 111-121 
    ISSN: 1573-3297
    Schlagwort(e): neurotransmitters ; genetics ; quantitative genetics ; pharmacology ; environment ; genetic correlations
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Psychologie
    Notizen: Abstract Genetic studies of neurotransmitter processes to date differ from most behavioral genetic studies in that the former have rarely considered individual variability, have tended to look only for single-gene effects, and generally have overlooked or ignored environmental influences. After examining these differences, we suggest that a rapprochement between the two fields will yield synergistic benefits in studies of the genetics of neuropharmacological processes as they affect behavior.
    Materialart: Digitale Medien
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 41 (1989), S. 23-29 
    ISSN: 1573-5060
    Schlagwort(e): Pelargonium x hortorum ; pelargonium ; flower doubleness ; nectary spur ; genetics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The formation of single flowers of 5 petals and 5 sepals is determined by the homozygous recessive state, dd, of the doubleness gene, D/d, which is epistatic to modifying genes determining flower type. In the presence of the dominant allele, i.e. genotypes DD or Dd, the flowers are semi-double or double. Owing to the D allele alone, the single frequency of 5 petals and 5 sepals is doubled to 10 petals and 10 sepals, of which up to 5 are petaloid, to give a semi-double flower. In addition, in the presence of the D allele, three modifying loci M1/m1, M2/m2, and M3/m3 are activated to give a series of distinct doubles with integral multiples of the basic perianth number. The homozygous recessive genes m1m1 and m2m2 both add an increment of 10 perianth parts, and m3m3 adds an increment of 20 perianth parts. In heterozygotes, M1m1, M2m2 and M3m3, the dominant alleles inhibit the incremental effect of their corresponding recessive alleles. The single flower cultivars investigated probably have the genotype dd, M1M1, M2M2, M3M3 and the semi-double cultivars the genotype Dd, M1m1, M2M2, M3M3. The single flowers have a nectariferous spur, characteristic of the genus, adnate to the pedicel. As the spur is absent from semi-double and double flowers, its presence is assumed to be either a pleiotropic effect of the single flower gene, or to be controlled by an unidentified gene tightly linked with it.
    Materialart: Digitale Medien
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 30 (1981), S. 881-887 
    ISSN: 1573-5060
    Schlagwort(e): Pelargonium × hortorum ; Pelargonium ; fertilization ; embryo survival ; plastid crosses ; genetics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The 6×6×4 analysis of variance of the mean percentage embryo survival for six variegated cultivars of Pelargonium × hortorum Bailey, with mutant plastids in their germ layers, and for their isogenic green (G) clones, shows highly significant differences between females (81 to 91 per cent) and between plastid crosses (83 to 91 per cent). Between cultivar differences are attributed largely to additive effects corresponding to additive gene action, and between plastid differences to a lower survival after crosses with white (W) males than with green males. The relationship between overall fertility (% fertilization × survival) and plastid crosses is a stepwise decline in the order G×G〉G×W〉W×G〉W×W (28 to 19 per cent) in which the white embryos growing in a white mother are approximately 30 per cent less fertile than the green embryos in a green mother. The non-surviving embryos are classified into empty, undeveloped and dwarf embryos and submitted to a 6×4 analysis of variance after summing through males. The significant heterogeneity between females is attributed largely to a difference between one cultivar-with a high frequency of dwarf and a low frequency of undeveloped embryos-and the other five cultivars. The absence of a significant plastid effect upon any stage of embryo breakdown indicates that the depressive effect of white plastids is spread evenly throughout development.
    Materialart: Digitale Medien
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 33 (1984), S. 169-176 
    ISSN: 1573-5060
    Schlagwort(e): Mirabilis jalapa ; marvel of Peru ; four o'clock ; leaf variegation ; mutable genes ; controlling elements ; genetics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The recurrent back-mutation from homozygous recessive chlorina to heterozygous dominant green in leaves of variegated Mirabilis jalapa is explicable on the hypothesis of a controlling element system. The analysis of variance of mutation frequency suggests that there is polygenic control modifying the major regulatory gene, an idea which is supported by a significant parent offspring regression. There is also evidence for a change of state in the regulatory element giving rise to a new mutability pattern.
    Materialart: Digitale Medien
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  • 6
    ISSN: 1573-3297
    Schlagwort(e): Quantitative trait locus ; recombinant inbred strains ; locomotor activity ; mouse ; genetics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Psychologie
    Notizen: Abstract The finding that stress-induced locomotor activity exhibited a significant strain × time interaction in the LS × SS RI strains prompted examination of QTL influencing this behavior as a function of time. The degree of genetic determination for locomotor activity was 0.26 for the first 5 min and decreased to 0.16 for the last 5 min of a 30-min test but the number of genetic factors stayed relatively constant (three or four) across time. A QTL point analysis revealed a total of 15 QTL, 5–8 per 5-min time block. Few of the QTL were detected across all time points and different combinations of QTL were evident for each time period. Five of the QTL were in common with those reported by other investigators for similar behaviors. The results suggest that locomotor behavior is under a greater degree of genetic control during the initial part of the test but environmental factors become increasingly important as the test progresses. Furthermore, different genetic factors appear to be mediating genetic variation in locomotor behavior at any given time point.
    Materialart: Digitale Medien
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  • 7
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Proteins: Structure, Function, and Genetics 22 (1995), S. 413-418 
    ISSN: 0887-3585
    Schlagwort(e): protein folding ; lattice models ; protein energetics ; local interactions ; spin-glass theory ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: Lattice models of proteins were used to examine the role of local propensities in stabilizing the native state of a protein, using techniques drawn from spin-glass theory to characterize the free-energy landscapes. In the strong evolutionary limit, optimal conditions for folding are achieved when the contributions from local interactions to the stability of the native state is small. Further increasing the local interactions rapidly decreases the foldability. © 1995 Wiley-Liss, Inc.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
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  • 8
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Proteins: Structure, Function, and Genetics 25 (1996), S. 38-47 
    ISSN: 0887-3585
    Schlagwort(e): protein structure prediction ; Bayesian statistics ; amino acid substitution ; information theory ; solvent accessibility ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: We introduce a novel Bayesian probabilistic method for predicting the solvent accessibilities of amino acid residues in globular proteins. Using single sequence data, this method achieves prediction accuracies higher than previously published methods. Substantially improved predictions - comparable to the highest accuracies reported in the literature to date - are obtained by representing alignments of the example proteins and their homologs as strings of residue substitution classes, depending on the side chain types observed at each alignment position. These results demonstrate the applicability of this relatively simple Bayesian approach to structure prediction and illustrate the utility of the classification methodology previously developed to extract information from aligned sets of structurally related proteins. © 1996 Wiley-Liss, Inc.
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
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  • 9
    Digitale Medien
    Digitale Medien
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 15 (1988), S. 193-204 
    ISSN: 0887-6134
    Schlagwort(e): Chemistry ; Analytical Chemistry and Spectroscopy
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: A method which involves the use of tandem mass spectrometry (MS/MS) for the identification of drug metabolites has been demonstrated with a triple quadrupole mass spectrometer. The method is based on the fact that metabolites usually retain various substructures of the original drug molecule. MS/MS is capable of rapidly identifying molecules with characteristic substructures without prior separation. It is shown that this method makes it possible to postulate possible drug metabolite structures rapidly and systematically without the use of standards. The MS/MS method, as it was applied to the identification of the metabolites of a new antiepileptic drug, zonisamide, is discussed. In this case it was possible to identify isomeric metabolites due to their differences in vaporization times off the probe and their different daughter spectra. The complementary uses of the neutral loss and parent scans for the determination of the site of metabolism is demonstrated. A new figure of merit, the limit of identification, is introduced. The amount of the epoxide metabolite of carbamazepine necessary for its reliable identification in urine was shown to be 0.4 ng/μl. The application of various techniques to confirm preliminary findings with this MS/MS method are described.
    Zusätzliches Material: 12 Ill.
    Materialart: Digitale Medien
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  • 10
    Digitale Medien
    Digitale Medien
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 32 (1994), S. 2049-2056 
    ISSN: 0887-624X
    Schlagwort(e): metallocenes ; olefin polymerizations ; Ziegler-Natta catalysts ; Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: Propylene was polymerized with rac-ethylene-bis (1-η5-indenyl)dichlorozirconium/methylaluminoxane in solvents of different polarity. The poly (propylene) formed was separated by solvent extraction; 13C-NMR and DSC measurements were made on the polymer fractions. The poly(propylene) in each solvent fraction has its characteristic molecular weight steric pentad distributions, melting transition temperature, and enthalpy for fusion irrespective of the polymerization medium. The results suggest that the medium dielectric constant does not affect the polymerization rate or the intrinsic stereoselectivity, propagation and chain transfer rates a given catalytic species but can alter the occurrence of steric insertion errors through shifting of distributions of the propagating species producing poly(propylenes) of different stereoregularities. © 1994 John Wiley & Sons, Inc.
    Zusätzliches Material: 7 Tab.
    Materialart: Digitale Medien
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