ISSN:
1432-0428
Keywords:
Spiny mice (Acomys cahirinus)
;
isolated islets
;
glucose
;
theophylline
;
arginine
;
cytochalasin B
;
vincristine
;
insulin releasein vitro
;
sensitivity to glucose
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Immunoreactive insulin (IRI) release from collagenase isolated pancreatic islets of spiny mice (Acomys cahirinus) was examined in 15 min and 2 h incubations at glucose concentrations between 2.8 and 56 mM. The resultant glucose-insulin dose-response curves forAcomys islets were compared to those for similarly incubated rat islets. After 15 min incubations, IRI release fromAcomys islets was significantly lower than that from rat islets at all stimulating glucose concentrations. After 2 h incubations, however, maximal responses were similar forAcomys and rat islets, whereas thesensitivity ofAcomys islets to glucose was significantly less. Cyclic AMP 10 mM, theophylline 5 mM, arginine 10 mM, and cytochalasin B 10 Μg/ml, all enhanced IRI release in the presence of glucose 16.7 mM to a relatively greater extent fromAcomys than from rat islets. Vincristine 10−5M reduced IRI release from bothAcomys and rat islets, and this to a similar extent. The results suggest that defective IRI release fromAcomys islets may be the expression of decreased B-cell sensitivity to glucose, even though IRI release fromAcomys islets may improve in time with continued exposure to elevated glucose concentrations. The ability of B-cells ofAcomys to recognize other agents that enhance the action of glucose appeared unaltered.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01222001
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